LOCUS       sequence-147549-       11110 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses dCas9-VP64 under actin promoter for SAM CRISPRa in 
            Drosophila cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11110)
  AUTHORS   Chavez A, Tuttle M, Pruitt BW, Ewen-Campen B, Chari R, Ter-Ovanesyan
            D, Haque SJ, Cecchi RJ, Kowal EJ, Buchthal J, Housden BE, Perrimon 
            N, Collins JJ, Church G
  TITLE     Comparison of Cas9 activators in multiple species.
  JOURNAL   Nat Methods. 2016 May 23. doi: 10.1038/nmeth.3871.
  PUBMED    27214048
REFERENCE   2  (bases 1 to 11110)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 May 23. doi: 10.1038/nmeth.3871."
COMMENT     Sequence Label: pAct:dCas9-VP64
FEATURES             Location/Qualifiers
     source          1..11110
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        140..2649
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     protein_bind    2667..2691
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2706..2753
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             join(2754..2777,2778..2780,2781..5336,5337..5339,
                     5340..6854)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the N863A mutation in the HNH 
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKARGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6909..6929
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6954..7103
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             7125..7178
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             7179..7577
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     protein_bind    complement(7579..7603)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      7611..7620
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(7617..7619,7620..7622,7623..8333)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(7617..7619,7620..7622,7623..8333)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7923..7942)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8270..8291
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     intron          8438..8503
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             8633..8653
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9078..9212
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9115..9134)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9169..9188
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             join(9309..9377,9378..10169)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9527..9546)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10340..10928
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10829..10848
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11082..11099
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcaggcaact
       61 cgtgaaaggt aggcggatca gcggccgcag catgcaattc tatattctaa aaacacaaat
      121 gatacttcta aaaaaaaatc atgaatggca tcaactctga atcaaatctt tgcagatgca
      181 cctacttctc atttccactg tcacatcatt tttccagatc tcgctgcctg ttatgtggcc
      241 cacaaaccaa gacacgtttt atggccatta aagctggctg atcgtcgcca aacaccaaat
      301 acataatgaa tatgtacaca ttcgagaaag aagcgatcaa agaagcgtct tcgggcggag
      361 taggagaatg cggaggagaa ggagaacgag ctgatctagt atctctccac aatccaatgc
      421 caactgacca actggccata ttcggagcaa tttgaagcca atttccatcg cctggcgatc
      481 gctccattct tggctatatg tttttcaccg ttacccgggg ccattttcaa agactcgtcg
      541 gcaagataag attgtgtcac tcgctgtctc tcttcatttg tcgaagaatg ctgaggaatt
      601 tcgcgatgac gtcggcgagt attttgaaga atgagaataa tttgtattta tacgaaaatc
      661 agttagtgga attttctaca aaaacatgtt atctatagat aattttgttg caaaatatgt
      721 tgactatgac aaagattgta tgtatatacc tttaatgtat tctcattttc ttatgtattt
      781 ataatggcaa tgatgatact gatgatattt taagatgatg ccagaccaaa aggcttgaat
      841 ttctgcgtct tttgccgaac gcagtgcatg tgcaattgtt gttttttgga atattcaatt
      901 ttcggactgt ccgctttgat ttcagtttct tggcttattc aaaaagcaaa gtaaagccaa
      961 aaaagcgaga tggcaatacc aaatgcggca aaacggtagt ggaaggaaag gggtgcgggg
     1021 cagcggaagg aagggtgggg cggggcgtgg cggggtctgt ggctgggcgc gacgtcaccg
     1081 acgttggagc cactcctttg accatgtgtg cgtgtgtgta ttattcgtgt ctcgccactc
     1141 gccggttgtt tttttctttt tatgctgcgc tctctctagc gccatctcgc ttacgcatgc
     1201 tcaacgcacc gcatgttgcc gtttcctttt atgcgtcatt ttggctcgaa ataggcaatt
     1261 atttaaacaa agattagtca acgaaaacgc taaaataaat aagtctacaa tatggttact
     1321 tattgccatg tgtgtgcagc caacgatagc aacaaaagca acaacacagg tggctttccc
     1381 tctttcactt tttgtttgca agccgcgtgc gagcaagacg gcacgaccgg caaacgcaat
     1441 tacgctgaca aagagcagac gaagttttgg cgaaaaacat caaggcgcct gatacgaatg
     1501 catttgcaat aacaattgcg atatttaata ttgtttatga agctgtttga cttcaaaaca
     1561 cacaaaaaaa aaaataaaac aaattatttg aaagagaatt aggaatcgga cgcttatcgt
     1621 tagggtaaca acaagaaatg cttactgagt cacagcctct ggaaaactgc cgcaagccag
     1681 agagagagag aaaaagaggg agagcagctt agaccgcatg tgcttgtgtg tgaggcgtct
     1741 ctctcttcgt ctctgttgcg caaacgcata gactgcactg agaaaatcga ttacctattt
     1801 tttatgaatg aatatttgca ctattactat tcaaaactat taagatagca atcacattca
     1861 atagccaaat actataccac ctgagcgatg caacgaaatg atcaatttga gcaaaaatgc
     1921 tgcatattta ggacggcatc attatagaaa tgcttcttgc tgtgtacttt tctctcgtct
     1981 ggcagctgtt tcgccgttat tgttaaaacc ggcttaagtt aggtgtgttt tctacgacta
     2041 gtgaatgccc tactagaaga tgtgtgttgc acaaaatgtc cctggaataa ccaatttgaa
     2101 gtgcagatag cagtaaacgt aagctaatat gaatattatt taactgtaat gttttaatat
     2161 cgctggacat tactaataaa cccactataa acacatgtac atatgtatgt tttggcatac
     2221 aatgagtagt tggggaaaaa atgtgtaaaa gcaccgtgac catcacagca taaagataac
     2281 cagctgaagt atcgaatatg agtaaccccc aaattgaatc acatgccgca actgatagga
     2341 cccatggaag tacactcttc atggcgatat acaagacaca cacaagcacg aacacccagt
     2401 tgcggaggaa attctccgta aatgaaaacc caatcggcga acaattcata cccatatatg
     2461 gtaaaagttt tgaacgcgac ttgagagcgg agagcattgc ggctgataag gttttagcgc
     2521 taagcgggct ttataaaacg ggctgcggga ccagttttca tatcactacc gtttgagttc
     2581 ttgtgctgtg tggatactcc tcccgacaca aagccgctcc atcagccagc agtcgtctaa
     2641 tccagagacc ccggatccag atatcgacaa gtttgtacaa aaaagcaggc tacaaagcca
     2701 ccatgaaaag gccggcggcc acgaaaaagg ccggccaggc aaaaaagaaa aaggacaaga
     2761 agtacagcat cggcctggcc atcggcacca actctgtggg ctgggccgtg atcaccgacg
     2821 agtacaaggt gcccagcaag aaattcaagg tgctgggcaa caccgaccgg cacagcatca
     2881 agaagaacct gatcggagcc ctgctgttcg acagcggcga aacagccgag gccacccggc
     2941 tgaagagaac cgccagaaga agatacacca gacggaagaa ccggatctgc tatctgcaag
     3001 agatcttcag caacgagatg gccaaggtgg acgacagctt cttccacaga ctggaagagt
     3061 ccttcctggt ggaagaggat aagaagcacg agcggcaccc catcttcggc aacatcgtgg
     3121 acgaggtggc ctaccacgag aagtacccca ccatctacca cctgagaaag aaactggtgg
     3181 acagcaccga caaggccgac ctgcggctga tctatctggc cctggcccac atgatcaagt
     3241 tccggggcca cttcctgatc gagggcgacc tgaaccccga caacagcgac gtggacaagc
     3301 tgttcatcca gctggtgcag acctacaacc agctgttcga ggaaaacccc atcaacgcca
     3361 gcggcgtgga cgccaaggcc atcctgtctg ccagactgag caagagcaga cggctggaaa
     3421 atctgatcgc ccagctgccc ggcgagaaga agaatggcct gttcggcaac ctgattgccc
     3481 tgagcctggg cctgaccccc aacttcaaga gcaacttcga cctggccgag gatgccaaac
     3541 tgcagctgag caaggacacc tacgacgacg acctggacaa cctgctggcc cagatcggcg
     3601 accagtacgc cgacctgttt ctggccgcca agaacctgtc cgacgccatc ctgctgagcg
     3661 acatcctgag agtgaacacc gagatcacca aggcccccct gagcgcctct atgatcaaga
     3721 gatacgacga gcaccaccag gacctgaccc tgctgaaagc tctcgtgcgg cagcagctgc
     3781 ctgagaagta caaagagatt ttcttcgacc agagcaagaa cggctacgcc ggctacattg
     3841 acggcggagc cagccaggaa gagttctaca agttcatcaa gcccatcctg gaaaagatgg
     3901 acggcaccga ggaactgctc gtgaagctga acagagagga cctgctgcgg aagcagcgga
     3961 ccttcgacaa cggcagcatc ccccaccaga tccacctggg agagctgcac gccattctgc
     4021 ggcggcagga agatttttac ccattcctga aggacaaccg ggaaaagatc gagaagatcc
     4081 tgaccttccg catcccctac tacgtgggcc ctctggccag gggaaacagc agattcgcct
     4141 ggatgaccag aaagagcgag gaaaccatca ccccctggaa cttcgaggaa gtggtggaca
     4201 agggcgcttc cgcccagagc ttcatcgagc ggatgaccaa cttcgataag aacctgccca
     4261 acgagaaggt gctgcccaag cacagcctgc tgtacgagta cttcaccgtg tataacgagc
     4321 tgaccaaagt gaaatacgtg accgagggaa tgagaaagcc cgccttcctg agcggcgagc
     4381 agaaaaaggc catcgtggac ctgctgttca agaccaaccg gaaagtgacc gtgaagcagc
     4441 tgaaagagga ctacttcaag aaaatcgagt gcttcgactc cgtggaaatc tccggcgtgg
     4501 aagatcggtt caacgcctcc ctgggcacat accacgatct gctgaaaatt atcaaggaca
     4561 aggacttcct ggacaatgag gaaaacgagg acattctgga agatatcgtg ctgaccctga
     4621 cactgtttga ggacagagag atgatcgagg aacggctgaa aacctatgcc cacctgttcg
     4681 acgacaaagt gatgaagcag ctgaagcggc ggagatacac cggctggggc aggctgagcc
     4741 ggaagctgat caacggcatc cgggacaagc agtccggcaa gacaatcctg gatttcctga
     4801 agtccgacgg cttcgccaac agaaacttca tgcagctgat ccacgacgac agcctgacct
     4861 ttaaagagga catccagaaa gcccaggtgt ccggccaggg cgatagcctg cacgagcaca
     4921 ttgccaatct ggccggcagc cccgccatta agaagggcat cctgcagaca gtgaaggtgg
     4981 tggacgagct cgtgaaagtg atgggccggc acaagcccga gaacatcgtg atcgaaatgg
     5041 ccagagagaa ccagaccacc cagaagggac agaagaacag ccgcgagaga atgaagcgga
     5101 tcgaagaggg catcaaagag ctgggcagcc agatcctgaa agaacacccc gtggaaaaca
     5161 cccagctgca gaacgagaag ctgtacctgt actacctgca gaatgggcgg gatatgtacg
     5221 tggaccagga actggacatc aaccggctgt ccgactacga tgtggaccac atcgtgcctc
     5281 agagctttct gaaggacgac tccatcgaca acaaggtgct gaccagaagc gacaaggccc
     5341 ggggcaagag cgacaacgtg ccctccgaag aggtcgtgaa gaagatgaag aactactggc
     5401 ggcagctgct gaacgccaag ctgattaccc agagaaagtt cgacaatctg accaaggccg
     5461 agagaggcgg cctgagcgaa ctggataagg ccggcttcat caagagacag ctggtggaaa
     5521 cccggcagat cacaaagcac gtggcacaga tcctggactc ccggatgaac actaagtacg
     5581 acgagaatga caagctgatc cgggaagtga aagtgatcac cctgaagtcc aagctggtgt
     5641 ccgatttccg gaaggatttc cagttttaca aagtgcgcga gatcaacaac taccaccacg
     5701 cccacgacgc ctacctgaac gccgtcgtgg gaaccgccct gatcaaaaag taccctaagc
     5761 tggaaagcga gttcgtgtac ggcgactaca aggtgtacga cgtgcggaag atgatcgcca
     5821 agagcgagca ggaaatcggc aaggctaccg ccaagtactt cttctacagc aacatcatga
     5881 actttttcaa gaccgagatt accctggcca acggcgagat ccggaagcgg cctctgatcg
     5941 agacaaacgg cgaaaccggg gagatcgtgt gggataaggg ccgggatttt gccaccgtgc
     6001 ggaaagtgct gagcatgccc caagtgaata tcgtgaaaaa gaccgaggtg cagacaggcg
     6061 gcttcagcaa agagtctatc ctgcccaaga ggaacagcga taagctgatc gccagaaaga
     6121 aggactggga ccctaagaag tacggcggct tcgacagccc caccgtggcc tattctgtgc
     6181 tggtggtggc caaagtggaa aagggcaagt ccaagaaact gaagagtgtg aaagagctgc
     6241 tggggatcac catcatggaa agaagcagct tcgagaagaa tcccatcgac tttctggaag
     6301 ccaagggcta caaagaagtg aaaaaggacc tgatcatcaa gctgcctaag tactccctgt
     6361 tcgagctgga aaacggccgg aagagaatgc tggcctctgc cggcgaactg cagaagggaa
     6421 acgaactggc cctgccctcc aaatatgtga acttcctgta cctggccagc cactatgaga
     6481 agctgaaggg ctcccccgag gataatgagc agaaacagct gtttgtggaa cagcacaagc
     6541 actacctgga cgagatcatc gagcagatca gcgagttctc caagagagtg atcctggccg
     6601 acgctaatct ggacaaagtg ctgtccgcct acaacaagca ccgggataag cccatcagag
     6661 agcaggccga gaatatcatc cacctgttta ccctgaccaa tctgggagcc cctgccgcct
     6721 tcaagtactt tgacaccacc atcgaccgga agaggtacac cagcaccaaa gaggtgctgg
     6781 acgccaccct gatccaccag agcatcaccg gcctgtacga gacacggatc gacctgtctc
     6841 agctgggagg cgacagcgct ggaggaggtg gaagcggagg aggaggaagc ggaggaggag
     6901 gtagcggacc taagaaaaag aggaaggtgg cggccgctgg atccggacgg gctgacgcat
     6961 tggacgattt tgatctggat atgctgggaa gtgacgccct cgatgatttt gaccttgaca
     7021 tgcttggttc ggatgccctt gatgactttg acctcgacat gctcggcagt gacgcccttg
     7081 atgatttcga cctggacatg ctgattaact gtacaggcag tggagagggc agaggaagtc
     7141 tgctaacatg cggtgacgtc gaggagaatc ctggcccaat ggccaagcct ttgtctcaag
     7201 aagaatccac cctcattgaa agagcaacgg ctacaatcaa cagcatcccc atctctgaag
     7261 actacagcgt cgccagcgca gctctctcta gcgacggccg catcttcact ggtgtcaatg
     7321 tatatcattt tactggggga ccttgtgcag aactcgtggt gctgggcact gctgctgctg
     7381 cggcagctgg caacctgact tgtatcgtcg cgatcggaaa tgagaacagg ggcatcttga
     7441 gcccctgcgg acggtgccga caggtgcttc tcgatctgca tcctgggatc aaagccatag
     7501 tgaaggacag tgatggacag ccgacggcag ttgggattcg tgaattgctg ccctctggtt
     7561 atgtgtggga gggctaaaac ccagctttct tgtacaaagt ggtgagctcc gccaccatgg
     7621 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
     7681 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
     7741 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
     7801 tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc
     7861 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
     7921 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
     7981 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
     8041 tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca
     8101 tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc
     8161 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
     8221 tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
     8281 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagcaccggt
     8341 ccacgtgacg taagctagca ggatctttgt gaaggaacct tacttctgtg gtgtgacata
     8401 attggacaaa ctacctacag agatttaaag ctctaaggta aatataaaat ttttaagtgt
     8461 ataatgtgtt aaactactga ttctaattgt ttgtgtattt tagattccaa cctatggaac
     8521 tgatgaatgg gagcagtggt ggaatgcctt taatgaggaa aacctgtttt gctcagaaga
     8581 aatgccatct agtgatgatg aggctactgc tgactctcaa cattctactc ctccaaaaaa
     8641 gaagagaaag gtagaagacc ccaaggactt tccttcagaa ttgctaagtt ttttgagtca
     8701 tgctgtgttt agtaatagaa ctcttgcttg ctttgctatt tacaccacaa aggaaaaagc
     8761 tgcactgcta tacaagaaaa ttatggaaaa atattctgta acctttataa gtaggcataa
     8821 cagttataat cataacatac tgttttttct tactccacac aggcatagag tgtctgctat
     8881 taataactat gctcaaaaat tgtgtacctt tagcttttta atttgtaaag gggttaataa
     8941 ggaatatttg atgtatagtg ccttgactag agatcataat cagccatacc acatttgtag
     9001 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
     9061 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     9121 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     9181 aactcatcaa tgtatcttat catgtctgga tcccgtttaa actacgcgta attcaaacag
     9241 ggttctggcg tcgttctcgt actgttttcc ccaggccagt gctttagcgt tattgaaaaa
     9301 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     9361 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     9421 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     9481 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     9541 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     9601 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     9661 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     9721 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     9781 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     9841 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     9901 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     9961 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
    10021 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
    10081 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
    10141 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
    10201 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
    10261 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
    10321 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
    10381 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
    10441 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
    10501 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
    10561 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
    10621 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
    10681 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
    10741 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
    10801 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
    10861 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
    10921 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
    10981 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
    11041 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
    11101 aagcggaaga
//
