LOCUS       sequence-147551-        8758 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses MS2-p65-HSF under actin promoter for SAM CRISPRa in 
            Drosophila cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8758)
  AUTHORS   Chavez A, Tuttle M, Pruitt BW, Ewen-Campen B, Chari R, Ter-Ovanesyan
            D, Haque SJ, Cecchi RJ, Kowal EJ, Buchthal J, Housden BE, Perrimon 
            N, Collins JJ, Church G
  TITLE     Comparison of Cas9 activators in multiple species.
  JOURNAL   Nat Methods. 2016 May 23. doi: 10.1038/nmeth.3871.
  PUBMED    27214048
REFERENCE   2  (bases 1 to 8758)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 May 23. doi: 10.1038/nmeth.3871."
COMMENT     Sequence Label: pAct:MS2-p65-HSF
FEATURES             Location/Qualifiers
     source          1..8758
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        140..2649
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     protein_bind    2667..2691
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      2697..2706
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2706..3092
                     /codon_start=1
                     /product="bacteriophage MS2 coat protein"
                     /label=MS2-N55K
                     /note="binds with high affinity to a specific stem-loop 
                     structure in the viral RNA (Lim et al., 1994)"
                     /translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
                     CSVRQSSAQKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
                     VKAMQGLLKDGNPIPSAIAANSGIY"
     CDS             3147..3167
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3183..3725
                     /codon_start=1
                     /product="C-terminal portion of the p65 subunit of mouse 
                     NF-kappa-B"
                     /label=p65
                     /translation="PSGQISNQALALAPSSAPVLAQTMVPSSAMVPLAQPPAPAPVLTP
                     GPPQSLSAPVPKSTQAGEGTLSEALLHLQFDADEDLGALLGNSTDPGVFTDLASVDNSE
                     FQQLLNQGVSMSHSTAEPMLMEYPEAITRLVTGSQRPPDPAPTPLGTSGLPNGLSGDED
                     FSSIADMDFSALLSQISS"
     CDS             3750..4121
                     /codon_start=1
                     /product="C-terminal activation domain from the human heat 
                     shock transcription factor HSF1"
                     /label=HSF1
                     /translation="GFSVDTSALLDLFSPSVTVPDMSLPDLDSSLASIQELLSPQEPPR
                     PPEAENSSPDSGKQLVHYTAQPLFLLDPGSVDTGSNDLPVLFELGEGSYFSEGDGFAED
                     PTISLLTGSEPPKAKDPTVS"
     CDS             4137..4190
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4194..5213
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     "
     protein_bind    complement(5227..5251)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      5259..5268
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5265..5267,5268..5270,5271..5981)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5265..5267,5268..5270,5271..5981)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5310..5331)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5571..5590)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5918..5939
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     intron          6086..6151
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             6281..6301
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    6726..6860
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6763..6782)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6817..6836
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             join(6957..7025,7026..7817)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7175..7194)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7988..8576
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8477..8496
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8730..8747
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcaggcaact
       61 cgtgaaaggt aggcggatca gcggccgcag catgcaattc tatattctaa aaacacaaat
      121 gatacttcta aaaaaaaatc atgaatggca tcaactctga atcaaatctt tgcagatgca
      181 cctacttctc atttccactg tcacatcatt tttccagatc tcgctgcctg ttatgtggcc
      241 cacaaaccaa gacacgtttt atggccatta aagctggctg atcgtcgcca aacaccaaat
      301 acataatgaa tatgtacaca ttcgagaaag aagcgatcaa agaagcgtct tcgggcggag
      361 taggagaatg cggaggagaa ggagaacgag ctgatctagt atctctccac aatccaatgc
      421 caactgacca actggccata ttcggagcaa tttgaagcca atttccatcg cctggcgatc
      481 gctccattct tggctatatg tttttcaccg ttacccgggg ccattttcaa agactcgtcg
      541 gcaagataag attgtgtcac tcgctgtctc tcttcatttg tcgaagaatg ctgaggaatt
      601 tcgcgatgac gtcggcgagt attttgaaga atgagaataa tttgtattta tacgaaaatc
      661 agttagtgga attttctaca aaaacatgtt atctatagat aattttgttg caaaatatgt
      721 tgactatgac aaagattgta tgtatatacc tttaatgtat tctcattttc ttatgtattt
      781 ataatggcaa tgatgatact gatgatattt taagatgatg ccagaccaaa aggcttgaat
      841 ttctgcgtct tttgccgaac gcagtgcatg tgcaattgtt gttttttgga atattcaatt
      901 ttcggactgt ccgctttgat ttcagtttct tggcttattc aaaaagcaaa gtaaagccaa
      961 aaaagcgaga tggcaatacc aaatgcggca aaacggtagt ggaaggaaag gggtgcgggg
     1021 cagcggaagg aagggtgggg cggggcgtgg cggggtctgt ggctgggcgc gacgtcaccg
     1081 acgttggagc cactcctttg accatgtgtg cgtgtgtgta ttattcgtgt ctcgccactc
     1141 gccggttgtt tttttctttt tatgctgcgc tctctctagc gccatctcgc ttacgcatgc
     1201 tcaacgcacc gcatgttgcc gtttcctttt atgcgtcatt ttggctcgaa ataggcaatt
     1261 atttaaacaa agattagtca acgaaaacgc taaaataaat aagtctacaa tatggttact
     1321 tattgccatg tgtgtgcagc caacgatagc aacaaaagca acaacacagg tggctttccc
     1381 tctttcactt tttgtttgca agccgcgtgc gagcaagacg gcacgaccgg caaacgcaat
     1441 tacgctgaca aagagcagac gaagttttgg cgaaaaacat caaggcgcct gatacgaatg
     1501 catttgcaat aacaattgcg atatttaata ttgtttatga agctgtttga cttcaaaaca
     1561 cacaaaaaaa aaaataaaac aaattatttg aaagagaatt aggaatcgga cgcttatcgt
     1621 tagggtaaca acaagaaatg cttactgagt cacagcctct ggaaaactgc cgcaagccag
     1681 agagagagag aaaaagaggg agagcagctt agaccgcatg tgcttgtgtg tgaggcgtct
     1741 ctctcttcgt ctctgttgcg caaacgcata gactgcactg agaaaatcga ttacctattt
     1801 tttatgaatg aatatttgca ctattactat tcaaaactat taagatagca atcacattca
     1861 atagccaaat actataccac ctgagcgatg caacgaaatg atcaatttga gcaaaaatgc
     1921 tgcatattta ggacggcatc attatagaaa tgcttcttgc tgtgtacttt tctctcgtct
     1981 ggcagctgtt tcgccgttat tgttaaaacc ggcttaagtt aggtgtgttt tctacgacta
     2041 gtgaatgccc tactagaaga tgtgtgttgc acaaaatgtc cctggaataa ccaatttgaa
     2101 gtgcagatag cagtaaacgt aagctaatat gaatattatt taactgtaat gttttaatat
     2161 cgctggacat tactaataaa cccactataa acacatgtac atatgtatgt tttggcatac
     2221 aatgagtagt tggggaaaaa atgtgtaaaa gcaccgtgac catcacagca taaagataac
     2281 cagctgaagt atcgaatatg agtaaccccc aaattgaatc acatgccgca actgatagga
     2341 cccatggaag tacactcttc atggcgatat acaagacaca cacaagcacg aacacccagt
     2401 tgcggaggaa attctccgta aatgaaaacc caatcggcga acaattcata cccatatatg
     2461 gtaaaagttt tgaacgcgac ttgagagcgg agagcattgc ggctgataag gttttagcgc
     2521 taagcgggct ttataaaacg ggctgcggga ccagttttca tatcactacc gtttgagttc
     2581 ttgtgctgtg tggatactcc tcccgacaca aagccgctcc atcagccagc agtcgtctaa
     2641 tccagagacc ccggatccag atatcgacaa gtttgtacaa aaaagcaggc tacaaagcca
     2701 ccatggcttc aaactttact cagttcgtgc tcgtggacaa tggtgggaca ggggatgtga
     2761 cagtggctcc ttctaatttc gctaatgggg tggcagagtg gatcagctcc aactcacgga
     2821 gccaggccta caaggtgaca tgcagcgtca ggcagtctag tgcccagaag agaaagtata
     2881 ccatcaaggt ggaggtcccc aaagtggcta cccagacagt gggcggagtc gaactgcctg
     2941 tcgccgcttg gaggtcctac ctgaacatgg agctcactat cccaattttc gctaccaatt
     3001 ctgactgtga actcatcgtg aaggcaatgc aggggctcct caaagacggt aatcctatcc
     3061 cttccgccat cgccgctaac tcaggtatct acagcgctgg aggaggtgga agcggaggag
     3121 gaggaagcgg aggaggaggt agcggaccta agaaaaagag gaaggtggcg gccgctggat
     3181 ccccttcagg gcagatcagc aaccaggccc tggctctggc ccctagctcc gctccagtgc
     3241 tggcccagac tatggtgccc tctagtgcta tggtgcctct ggcccagcca cctgctccag
     3301 cccctgtgct gaccccagga ccaccccagt cactgagcgc tccagtgccc aagtctacac
     3361 aggccggcga ggggactctg agtgaagctc tgctgcacct gcagttcgac gctgatgagg
     3421 acctgggagc tctgctgggg aacagcaccg atcccggagt gttcacagat ctggcctccg
     3481 tggacaactc tgagtttcag cagctgctga atcagggcgt gtccatgtct catagtacag
     3541 ccgaaccaat gctgatggag taccccgaag ccattacccg gctggtgacc ggcagccagc
     3601 ggccccccga ccccgctcca actcccctgg gaaccagcgg cctgcctaat gggctgtccg
     3661 gagatgaaga cttctcaagc atcgctgata tggactttag tgccctgctg tcacagattt
     3721 cctctagtgg gcagggagga ggtggaagcg gcttcagcgt ggacaccagt gccctgctgg
     3781 acctgttcag cccctcggtg accgtgcccg acatgagcct gcctgacctt gacagcagcc
     3841 tggccagtat ccaagagctc ctgtctcccc aggagccccc caggcctccc gaggcagaga
     3901 acagcagccc ggattcaggg aagcagctgg tgcactacac agcgcagccg ctgttcctgc
     3961 tggaccccgg ctccgtggac accgggagca acgacctgcc ggtgctgttt gagctgggag
     4021 agggctccta cttctccgaa ggggacggct tcgccgagga ccccaccatc tccctgctga
     4081 caggctcgga gcctcccaaa gccaaggacc ccactgtctc ctgtacaggc agtggagagg
     4141 gcagaggaag tctgctaaca tgcggtgacg tcgaggagaa tcctggccca accatgaaaa
     4201 agcctgaact caccgctacc tctgtcgaga agtttctgat cgaaaagttc gacagcgtct
     4261 ccgacctgat gcagctctcc gagggcgaag aatctcgggc tttcagcttc gatgtgggag
     4321 ggcgtggata tgtcctgcgg gtgaatagct gcgccgatgg tttctacaaa gatcgctatg
     4381 tttatcggca ctttgcatcc gccgctctcc ctattcccga agtgcttgac attggggagt
     4441 tcagcgagag cctgacctat tgcatctccc gccgtgcaca gggtgtcacc ttgcaagacc
     4501 tgcctgaaac cgaactgccc gctgttctcc agcccgtcgc cgaggccatg gatgccatcg
     4561 ctgccgccga tcttagccag accagcgggt tcggcccatt cggacctcaa ggaatcggtc
     4621 aatacactac atggcgcgat ttcatctgcg ctattgctga tccccatgtg tatcactggc
     4681 aaactgtgat ggacgacacc gtcagtgcct ccgtcgccca ggctctcgat gagctgatgc
     4741 tttgggccga ggactgcccc gaagtccggc acctcgtgca cgccgatttc ggctccaaca
     4801 atgtcctgac cgacaatggc cgcataacag ccgtcattga ctggagcgag gccatgttcg
     4861 gggattccca atacgaggtc gccaacatct tcttctggag gccctggttg gcttgtatgg
     4921 agcagcagac ccgctacttc gagcggaggc atcccgagct tgcaggatct cctcggctcc
     4981 gggcttatat gctccgcatt ggtcttgacc aactctatca gagcttggtt gacggcaatt
     5041 tcgatgatgc agcttgggct cagggtcgct gcgacgcaat cgtccggtcc ggagccggga
     5101 ctgtcgggcg tacacaaatc gcccgcagaa gcgctgccgt ctggaccgat ggctgtgtgg
     5161 aagtgctcgc cgatagtgga aacagacgcc ccagcactcg tcctagggca aaggatctgc
     5221 agtaaaaccc agctttcttg tacaaagtgg tgagctccgc caccatggtg agcaagggcg
     5281 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
     5341 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga
     5401 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga
     5461 cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca
     5521 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca
     5581 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
     5641 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact
     5701 acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact
     5761 tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga
     5821 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt
     5881 ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga
     5941 ccgccgccgg gatcactctc ggcatggacg agctgtacaa gcaccggtcc acgtgacgta
     6001 agctagcagg atctttgtga aggaacctta cttctgtggt gtgacataat tggacaaact
     6061 acctacagag atttaaagct ctaaggtaaa tataaaattt ttaagtgtat aatgtgttaa
     6121 actactgatt ctaattgttt gtgtatttta gattccaacc tatggaactg atgaatggga
     6181 gcagtggtgg aatgccttta atgaggaaaa cctgttttgc tcagaagaaa tgccatctag
     6241 tgatgatgag gctactgctg actctcaaca ttctactcct ccaaaaaaga agagaaaggt
     6301 agaagacccc aaggactttc cttcagaatt gctaagtttt ttgagtcatg ctgtgtttag
     6361 taatagaact cttgcttgct ttgctattta caccacaaag gaaaaagctg cactgctata
     6421 caagaaaatt atggaaaaat attctgtaac ctttataagt aggcataaca gttataatca
     6481 taacatactg ttttttctta ctccacacag gcatagagtg tctgctatta ataactatgc
     6541 tcaaaaattg tgtaccttta gctttttaat ttgtaaaggg gttaataagg aatatttgat
     6601 gtatagtgcc ttgactagag atcataatca gccataccac atttgtagag gttttacttg
     6661 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg
     6721 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     6781 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     6841 tatcttatca tgtctggatc ccgtttaaac tacgcgtaat tcaaacaggg ttctggcgtc
     6901 gttctcgtac tgttttcccc aggccagtgc tttagcgtta ttgaaaaagg aagagtatga
     6961 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     7021 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     7081 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     7141 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     7201 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     7261 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     7321 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     7381 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     7441 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     7501 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     7561 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     7621 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     7681 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     7741 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     7801 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     7861 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     7921 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     7981 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     8041 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     8101 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     8161 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     8221 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     8281 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     8341 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     8401 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     8461 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     8521 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     8581 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     8641 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     8701 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaaga
//
