LOCUS       sequence-147554-       10375 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses dCas9 under actin promoter for ""Scaffold"" CRISPRa in 
            Drosophila cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10375)
  AUTHORS   Chavez A, Tuttle M, Pruitt BW, Ewen-Campen B, Chari R, Ter-Ovanesyan
            D, Haque SJ, Cecchi RJ, Kowal EJ, Buchthal J, Housden BE, Perrimon 
            N, Collins JJ, Church G
  TITLE     Comparison of Cas9 activators in multiple species.
  JOURNAL   Nat Methods. 2016 May 23. doi: 10.1038/nmeth.3871.
  PUBMED    27214048
REFERENCE   2  (bases 1 to 10375)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 May 23. doi: 10.1038/nmeth.3871."
COMMENT     Sequence Label: pAct:dCas9
FEATURES             Location/Qualifiers
     source          1..10375
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        140..2649
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     protein_bind    2667..2691
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      2697..2706
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2703..2729,2730..2732,2733..5216,5217..5222,5223..5288,
                     5289..5291,5292..6806)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=Cas9m4
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the mutations D10A, D839A, 
                     H840A, and N863A (Mali et al., 2013)"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVAAIVPQSFLKDDSIDNKVLTRSDKARGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             6819..6839
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(6844..6868)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      6876..6885
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6882..6884,6885..6887,6888..7598)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6882..6884,6885..6887,6888..7598)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6927..6948)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7188..7207)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7535..7556
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     intron          7703..7768
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             7898..7918
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8343..8477
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8380..8399)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8434..8453
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             join(8574..8642,8643..9434)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8792..8811)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9605..10193
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10094..10113
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10347..10364
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcaggcaact
       61 cgtgaaaggt aggcggatca gcggccgcag catgcaattc tatattctaa aaacacaaat
      121 gatacttcta aaaaaaaatc atgaatggca tcaactctga atcaaatctt tgcagatgca
      181 cctacttctc atttccactg tcacatcatt tttccagatc tcgctgcctg ttatgtggcc
      241 cacaaaccaa gacacgtttt atggccatta aagctggctg atcgtcgcca aacaccaaat
      301 acataatgaa tatgtacaca ttcgagaaag aagcgatcaa agaagcgtct tcgggcggag
      361 taggagaatg cggaggagaa ggagaacgag ctgatctagt atctctccac aatccaatgc
      421 caactgacca actggccata ttcggagcaa tttgaagcca atttccatcg cctggcgatc
      481 gctccattct tggctatatg tttttcaccg ttacccgggg ccattttcaa agactcgtcg
      541 gcaagataag attgtgtcac tcgctgtctc tcttcatttg tcgaagaatg ctgaggaatt
      601 tcgcgatgac gtcggcgagt attttgaaga atgagaataa tttgtattta tacgaaaatc
      661 agttagtgga attttctaca aaaacatgtt atctatagat aattttgttg caaaatatgt
      721 tgactatgac aaagattgta tgtatatacc tttaatgtat tctcattttc ttatgtattt
      781 ataatggcaa tgatgatact gatgatattt taagatgatg ccagaccaaa aggcttgaat
      841 ttctgcgtct tttgccgaac gcagtgcatg tgcaattgtt gttttttgga atattcaatt
      901 ttcggactgt ccgctttgat ttcagtttct tggcttattc aaaaagcaaa gtaaagccaa
      961 aaaagcgaga tggcaatacc aaatgcggca aaacggtagt ggaaggaaag gggtgcgggg
     1021 cagcggaagg aagggtgggg cggggcgtgg cggggtctgt ggctgggcgc gacgtcaccg
     1081 acgttggagc cactcctttg accatgtgtg cgtgtgtgta ttattcgtgt ctcgccactc
     1141 gccggttgtt tttttctttt tatgctgcgc tctctctagc gccatctcgc ttacgcatgc
     1201 tcaacgcacc gcatgttgcc gtttcctttt atgcgtcatt ttggctcgaa ataggcaatt
     1261 atttaaacaa agattagtca acgaaaacgc taaaataaat aagtctacaa tatggttact
     1321 tattgccatg tgtgtgcagc caacgatagc aacaaaagca acaacacagg tggctttccc
     1381 tctttcactt tttgtttgca agccgcgtgc gagcaagacg gcacgaccgg caaacgcaat
     1441 tacgctgaca aagagcagac gaagttttgg cgaaaaacat caaggcgcct gatacgaatg
     1501 catttgcaat aacaattgcg atatttaata ttgtttatga agctgtttga cttcaaaaca
     1561 cacaaaaaaa aaaataaaac aaattatttg aaagagaatt aggaatcgga cgcttatcgt
     1621 tagggtaaca acaagaaatg cttactgagt cacagcctct ggaaaactgc cgcaagccag
     1681 agagagagag aaaaagaggg agagcagctt agaccgcatg tgcttgtgtg tgaggcgtct
     1741 ctctcttcgt ctctgttgcg caaacgcata gactgcactg agaaaatcga ttacctattt
     1801 tttatgaatg aatatttgca ctattactat tcaaaactat taagatagca atcacattca
     1861 atagccaaat actataccac ctgagcgatg caacgaaatg atcaatttga gcaaaaatgc
     1921 tgcatattta ggacggcatc attatagaaa tgcttcttgc tgtgtacttt tctctcgtct
     1981 ggcagctgtt tcgccgttat tgttaaaacc ggcttaagtt aggtgtgttt tctacgacta
     2041 gtgaatgccc tactagaaga tgtgtgttgc acaaaatgtc cctggaataa ccaatttgaa
     2101 gtgcagatag cagtaaacgt aagctaatat gaatattatt taactgtaat gttttaatat
     2161 cgctggacat tactaataaa cccactataa acacatgtac atatgtatgt tttggcatac
     2221 aatgagtagt tggggaaaaa atgtgtaaaa gcaccgtgac catcacagca taaagataac
     2281 cagctgaagt atcgaatatg agtaaccccc aaattgaatc acatgccgca actgatagga
     2341 cccatggaag tacactcttc atggcgatat acaagacaca cacaagcacg aacacccagt
     2401 tgcggaggaa attctccgta aatgaaaacc caatcggcga acaattcata cccatatatg
     2461 gtaaaagttt tgaacgcgac ttgagagcgg agagcattgc ggctgataag gttttagcgc
     2521 taagcgggct ttataaaacg ggctgcggga ccagttttca tatcactacc gtttgagttc
     2581 ttgtgctgtg tggatactcc tcccgacaca aagccgctcc atcagccagc agtcgtctaa
     2641 tccagagacc ccggatccag atatcgacaa gtttgtacaa aaaagcaggc tacaaagcca
     2701 ccatggacaa gaagtactcc attgggctcg ctatcggcac aaacagcgtc ggctgggccg
     2761 tcattacgga cgagtacaag gtgccgagca aaaaattcaa agttctgggc aataccgatc
     2821 gccacagcat aaagaagaac ctcattggcg ccctcctgtt cgactccggg gagacggccg
     2881 aagccacgcg gctcaaaaga acagcacggc gcagatatac ccgcagaaag aatcggatct
     2941 gctacctgca ggagatcttt agtaatgaga tggctaaggt ggatgactct ttcttccata
     3001 ggctggagga gtcctttttg gtggaggagg ataaaaagca cgagcgccac ccaatctttg
     3061 gcaatatcgt ggacgaggtg gcgtaccatg aaaagtaccc aaccatatat catctgagga
     3121 agaagcttgt agacagtact gataaggctg acttgcggtt gatctatctc gcgctggcgc
     3181 atatgatcaa atttcgggga cacttcctca tcgaggggga cctgaaccca gacaacagcg
     3241 atgtcgataa actctttatc caactggttc agacttacaa tcagcttttc gaagagaacc
     3301 cgatcaacgc atccggagtt gacgccaaag caatcctgag cgctaggctg tccaaatccc
     3361 ggcggctcga aaacctcatc gcacagctcc ctggggagaa gaagaacggc ctgtttggta
     3421 atcttatcgc cctgtcactc gggctgaccc ccaactttaa atctaacttc gacctggccg
     3481 aagatgccaa gcttcaactg agcaaagaca cctacgatga tgatctcgac aatctgctgg
     3541 cccagatcgg cgaccagtac gcagaccttt ttttggcggc aaagaacctg tcagacgcca
     3601 ttctgctgag tgatattctg cgagtgaaca cggagatcac caaagctccg ctgagcgcta
     3661 gtatgatcaa gcgctatgat gagcaccacc aagacttgac tttgctgaag gcccttgtca
     3721 gacagcaact gcctgagaag tacaaggaaa ttttcttcga tcagtctaaa aatggctacg
     3781 ccggatacat tgacggcgga gcaagccagg aggaatttta caaatttatt aagcccatct
     3841 tggaaaaaat ggacggcacc gaggagctgc tggtaaagct taacagagaa gatctgttgc
     3901 gcaaacagcg cactttcgac aatggaagca tcccccacca gattcacctg ggcgaactgc
     3961 acgctatcct caggcggcaa gaggatttct accccttttt gaaagataac agggaaaaga
     4021 ttgagaaaat cctcacattt cggataccct actatgtagg ccccctcgcc cggggaaatt
     4081 ccagattcgc gtggatgact cgcaaatcag aagagaccat cactccctgg aacttcgagg
     4141 aagtcgtgga taagggggcc tctgcccagt ccttcatcga aaggatgact aactttgata
     4201 aaaatctgcc taacgaaaag gtgcttccta aacactctct gctgtacgag tacttcacag
     4261 tttataacga gctcaccaag gtcaaatacg tcacagaagg gatgagaaag ccagcattcc
     4321 tgtctggaga gcagaagaaa gctatcgtgg acctcctctt caagacgaac cggaaagtta
     4381 ccgtgaaaca gctcaaagaa gactatttca aaaagattga atgtttcgac tctgttgaaa
     4441 tcagcggagt ggaggatcgc ttcaacgcat ccctgggaac gtatcacgat ctcctgaaaa
     4501 tcattaaaga caaggacttc ctggacaatg aggagaacga ggacattctt gaggacattg
     4561 tcctcaccct tacgttgttt gaagataggg agatgattga agaacgcttg aaaacttacg
     4621 ctcatctctt cgacgacaaa gtcatgaaac agctcaagag gcgccgatat acaggatggg
     4681 ggcggctgtc aagaaaactg atcaatggga tccgagacaa gcagagtgga aagacaatcc
     4741 tggattttct taagtccgat ggatttgcca accggaactt catgcagttg atccatgatg
     4801 actctctcac ctttaaggag gacatccaga aagcacaagt ttctggccag ggggacagtc
     4861 ttcacgagca catcgctaat cttgcaggta gcccagctat caaaaaggga atactgcaga
     4921 ccgttaaggt cgtggatgaa ctcgtcaaag taatgggaag gcataagccc gagaatatcg
     4981 ttatcgagat ggcccgagag aaccaaacta cccagaaggg acagaagaac agtagggaaa
     5041 ggatgaagag gattgaagag ggtataaaag aactggggtc ccaaatcctt aaggaacacc
     5101 cagttgaaaa cacccagctt cagaatgaga agctctacct gtactacctg cagaacggca
     5161 gggacatgta cgtggatcag gaactggaca tcaatcggct ctccgactac gacgtggctg
     5221 ctatcgtgcc ccagtctttt ctcaaagatg attctattga taataaagtg ttgacaagat
     5281 ccgataaagc tagagggaag agtgataacg tcccctcaga agaagttgtc aagaaaatga
     5341 aaaattattg gcggcagctg ctgaacgcca aactgatcac acaacggaag ttcgataatc
     5401 tgactaaggc tgaacgaggt ggcctgtctg agttggataa agccggcttc atcaaaaggc
     5461 agcttgttga gacacgccag atcaccaagc acgtggccca aattctcgat tcacgcatga
     5521 acaccaagta cgatgaaaat gacaaactga ttcgagaggt gaaagttatt actctgaagt
     5581 ctaagctggt ctcagatttc agaaaggact ttcagtttta taaggtgaga gagatcaaca
     5641 attaccacca tgcgcatgat gcctacctga atgcagtggt aggcactgca cttatcaaaa
     5701 aatatcccaa gcttgaatct gaatttgttt acggagacta taaagtgtac gatgttagga
     5761 aaatgatcgc aaagtctgag caggaaatag gcaaggccac cgctaagtac ttcttttaca
     5821 gcaatattat gaattttttc aagaccgaga ttacactggc caatggagag attcggaagc
     5881 gaccacttat cgaaacaaac ggagaaacag gagaaatcgt gtgggacaag ggtagggatt
     5941 tcgcgacagt ccggaaggtc ctgtccatgc cgcaggtgaa catcgttaaa aagaccgaag
     6001 tacagaccgg aggcttctcc aaggaaagta tcctcccgaa aaggaacagc gacaagctga
     6061 tcgcacgcaa aaaagattgg gaccccaaga aatacggcgg attcgattct cctacagtcg
     6121 cttacagtgt actggttgtg gccaaagtgg agaaagggaa gtctaaaaaa ctcaaaagcg
     6181 tcaaggaact gctgggcatc acaatcatgg agcgatcaag cttcgaaaaa aaccccatcg
     6241 actttctcga ggcgaaagga tataaagagg tcaaaaaaga cctcatcatt aagcttccca
     6301 agtactctct ctttgagctt gaaaacggcc ggaaacgaat gctcgctagt gcgggcgagc
     6361 tgcagaaagg taacgagctg gcactgccct ctaaatacgt taatttcttg tatctggcca
     6421 gccactatga aaagctcaaa gggtctcccg aagataatga gcagaagcag ctgttcgtgg
     6481 aacaacacaa acactacctt gatgagatca tcgagcaaat aagcgaattc tccaaaagag
     6541 tgatcctcgc cgacgctaac ctcgataagg tgctttctgc ttacaataag cacagggata
     6601 agcccatcag ggagcaggca gaaaacatta tccacttgtt tactctgacc aacttgggcg
     6661 cgcctgcagc cttcaagtac ttcgacacca ccatagacag aaagcggtac acctctacaa
     6721 aggaggtcct ggacgccaca ctgattcatc agtcaattac ggggctctat gaaacaagaa
     6781 tcgacctctc tcagctcggt ggagacagca gggctgaccc caagaagaag aggaaggtgt
     6841 aaaacccagc tttcttgtac aaagtggtga gctccgccac catggtgagc aagggcgagg
     6901 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
     6961 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
     7021 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct
     7081 acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
     7141 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
     7201 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
     7261 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
     7321 acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca
     7381 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
     7441 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg
     7501 ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
     7561 ccgccgggat cactctcggc atggacgagc tgtacaagca ccggtccacg tgacgtaagc
     7621 tagcaggatc tttgtgaagg aaccttactt ctgtggtgtg acataattgg acaaactacc
     7681 tacagagatt taaagctcta aggtaaatat aaaattttta agtgtataat gtgttaaact
     7741 actgattcta attgtttgtg tattttagat tccaacctat ggaactgatg aatgggagca
     7801 gtggtggaat gcctttaatg aggaaaacct gttttgctca gaagaaatgc catctagtga
     7861 tgatgaggct actgctgact ctcaacattc tactcctcca aaaaagaaga gaaaggtaga
     7921 agaccccaag gactttcctt cagaattgct aagttttttg agtcatgctg tgtttagtaa
     7981 tagaactctt gcttgctttg ctatttacac cacaaaggaa aaagctgcac tgctatacaa
     8041 gaaaattatg gaaaaatatt ctgtaacctt tataagtagg cataacagtt ataatcataa
     8101 catactgttt tttcttactc cacacaggca tagagtgtct gctattaata actatgctca
     8161 aaaattgtgt acctttagct ttttaatttg taaaggggtt aataaggaat atttgatgta
     8221 tagtgccttg actagagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt
     8281 taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg
     8341 ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     8401 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     8461 cttatcatgt ctggatcccg tttaaactac gcgtaattca aacagggttc tggcgtcgtt
     8521 ctcgtactgt tttccccagg ccagtgcttt agcgttattg aaaaaggaag agtatgagta
     8581 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     8641 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     8701 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     8761 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     8821 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     8881 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     8941 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     9001 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     9061 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     9121 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     9181 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     9241 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     9301 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     9361 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     9421 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     9481 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     9541 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     9601 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     9661 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     9721 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     9781 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     9841 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     9901 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     9961 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
    10021 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
    10081 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
    10141 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
    10201 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
    10261 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
    10321 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaaga
//
