LOCUS       sequence-147572-         785 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of human CLN3 with C terminal GFP fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 785)
  TITLE     Braulke lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pcDNA3.1 CLN3-GFP
FEATURES             Location/Qualifiers
     source          1..785
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1..10
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(34..53)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(35..53)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(98..301)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(127..147)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        302..681
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 ccatggtggc gacggggtac caagcttggg tctccctata gtgagtcgta ttaatttcga
       61 taagccagta agcagtgggt tctctagtta gccagagagc tctgcttata tagacctccc
      121 accgtacacg cctaccgccc atttgcgtca atggggcgga gttgttacga cattttggaa
      181 agtcccgttg attttggtgc caaaacaaac tcccattgac gtcaatgggg tggagacttg
      241 gaaatccccg tgagtcaaac cgctatccac gcccattgat gtactgccaa aaccgcatca
      301 ccatggtaat agcgatgact aatacgtaga tgtactgcca agtaggaaag tcccataagg
      361 tcatgtactg ggcataatgc caggcgggcc atttaccgtc attgacgtca atagggggcg
      421 tacttggcat atgatacact tgatgtactg ccaagtgggc agtttaccgt aaatactcca
      481 cccattgacg tcaatggaaa gtccctattg gcgttactat gggaacatac gtcattattg
      541 acgtcaatgg gcgggggtcg ttgggcggtc agccaggcgg gccatttacc gtaagttatg
      601 taacgcggaa ctccatatat gggctatgaa ctaatgaccc cgtaattgat tactattaat
      661 aactagtcaa taatcaatgt caacgcgtat atctggcccg tacatcgcga agcagcgcaa
      721 aacgcctaac cctaagcaga ttcttcatgc aattgtcggt caagccttgc cttgttgtag
      781 cttaa
//
