LOCUS       sequence-147577-         845 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  1 Part of 4-part switch.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Sun ZZ, Yeung E, Hayes CA, Noireaux V, Murray RM
  TITLE     Linear DNA for rapid prototyping of synthetic biological circuits in
            an Escherichia coli based TX-TL cell-free system.
  JOURNAL   ACS Synth Biol. 2014 Jun 20;3(6):387-97. doi: 10.1021/sb400131a. 
            Epub 2013 Dec 4.
  PUBMED    24303785
REFERENCE   2  (bases 1 to 845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb400131a";
            journalName: "ACS Synth Biol"; date: "2014-06-20- 20"; volume: "3"; 
            issue: "6"; pages: "387-97"
COMMENT     Sequence Label: P3U2C5T2-v1-2 (Pl-lacO1-tetR) (136p)
FEATURES             Location/Qualifiers
     source          1..845
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(128..751)
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     659..678
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     RBS             758..780
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    806..822
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 cgatatccgc ctgatgcgtg aacgtgacgg acgtaacgaa gacaaggcac gactctcaag
       61 ggcatcggtc gacacagaaa agcccgcctt tcggcgggct ttgctcgagt tattcaccag
      121 agcttcatta agacccactt tcacatttaa gttgtttttc taatccgcat atgatcaatt
      181 caaggccgaa taagaaggct ggctctgcac cttggtgatc aaataattcg atagcttgtc
      241 gtaataatgg cggcatacta tcagtagtag gtgtttccct ttcttcttta gcgacttgat
      301 gctcttgatc ttccaatacg caacctaaag taaaatgccc cacagcgctg agtgcatata
      361 atgcattctc tagtgaaaaa ccttgttggc ataaaaaggc taattgattt tcgagagttt
      421 catactgttt ttctgtaggc cgtgtaccta aatgtacttt tgctccatcg cgatgactta
      481 gtaaagcaca tctaaaactt ttagcgttat tacgtaaaaa atcttgccag ctttcccctt
      541 ctaaagggca aaagtgagta tggtgcctat ctaacatctc aatggctaag gcgtcgagca
      601 aagcccgctt attttttaca tgccaataca atgtaggctg ctctacacct agcttctggg
      661 cgagtttacg ggttgttaaa ccttcgattc cgacctcatt aagcagctct aatgcgctgt
      721 taatcacttt acttttatct aatctagaca tggtatatct ccttcttaaa gttaaacaaa
      781 attattgctt gctgtgctca gtatcttgtt atccgctcac aatgtcaatg ttatccgctc
      841 acatt
//
