LOCUS       sequence-147578-         937 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  1 Part of 4-part switch.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 937)
  AUTHORS   Sun ZZ, Yeung E, Hayes CA, Noireaux V, Murray RM
  TITLE     Linear DNA for rapid prototyping of synthetic biological circuits in
            an Escherichia coli based TX-TL cell-free system.
  JOURNAL   ACS Synth Biol. 2014 Jun 20;3(6):387-97. doi: 10.1021/sb400131a. 
            Epub 2013 Dec 4.
  PUBMED    24303785
REFERENCE   2  (bases 1 to 937)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb400131a";
            journalName: "ACS Synth Biol"; date: "2014-06-20- 20"; volume: "3"; 
            issue: "6"; pages: "387-97"
COMMENT     Sequence Label: P3U2C5T2-v1-2 (Pl-lacO1-tetR) (136p)
FEATURES             Location/Qualifiers
     source          1..937
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    111..127
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             153..175
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             182..805
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(255..274)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
ORIGIN
        1 tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagcga agacaacata
       61 gcattgctgt tccgctgggc atgcataaat gtgagcggat aacattgaca ttgtgagcgg
      121 ataacaagat actgagcaca gcaagcaata attttgttta actttaagaa ggagatatac
      181 catgtctaga ttagataaaa gtaaagtgat taacagcgca ttagagctgc ttaatgaggt
      241 cggaatcgaa ggtttaacaa cccgtaaact cgcccagaag ctaggtgtag agcagcctac
      301 attgtattgg catgtaaaaa ataagcgggc tttgctcgac gccttagcca ttgagatgtt
      361 agataggcac catactcact tttgcccttt agaaggggaa agctggcaag attttttacg
      421 taataacgct aaaagtttta gatgtgcttt actaagtcat cgcgatggag caaaagtaca
      481 tttaggtaca cggcctacag aaaaacagta tgaaactctc gaaaatcaat tagccttttt
      541 atgccaacaa ggtttttcac tagagaatgc attatatgca ctcagcgctg tggggcattt
      601 tactttaggt tgcgtattgg aagatcaaga gcatcaagtc gctaaagaag aaagggaaac
      661 acctactact gatagtatgc cgccattatt acgacaagct atcgaattat ttgatcacca
      721 aggtgcagag ccagccttct tattcggcct tgaattgatc atatgcggat tagaaaaaca
      781 acttaaatgt gaaagtgggt cttaatgaag ctctggtgaa taactcgagc aaagcccgcc
      841 gaaaggcggg cttttctgtg tcgaccgatg cccttgagag tcgtgccttg tcttcgttac
      901 gtccgtcacg ttcacgcatc aggcggatat cgttagc
//
