LOCUS       sequence-147588-         901 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  1 Part of 4-part switch.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Sun ZZ, Yeung E, Hayes CA, Noireaux V, Murray RM
  TITLE     Linear DNA for rapid prototyping of synthetic biological circuits in
            an Escherichia coli based TX-TL cell-free system.
  JOURNAL   ACS Synth Biol. 2014 Jun 20;3(6):387-97. doi: 10.1021/sb400131a. 
            Epub 2013 Dec 4.
  PUBMED    24303785
REFERENCE   2  (bases 1 to 901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb400131a";
            journalName: "ACS Synth Biol"; date: "2014-06-20- 20"; volume: "3"; 
            issue: "6"; pages: "387-97"
COMMENT     Sequence Label: P4U2C6T2-v1-2 (Pl-tetO1-lacI) (137p)
FEATURES             Location/Qualifiers
     source          1..901
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    91..109
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    116..134
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             158..180
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(206..225)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 acacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagcgaagac
       61 aacatagcat tgctgttccg ctgggcatgc tccctatcag tgatagagat tgacatccct
      121 atcagtgata gagatactga gcacagcaag caataatttt gtttaacttt aagaaggaga
      181 tataccatga aaccagtaac gttatacgat gtcgcagagt atgccggtgt ctcttatcag
      241 accgtttccc gcgtggtgaa ccaggccagc cacgtttctg cgaaaacgcg ggaaaaagtg
      301 gaagcggcga tggcggagct gaattacatt cccaaccgcg tggcacaaca actggcgggc
      361 aaacagtcgt tgctgattgg cgttgccacc tccagtctgg ccctgcacgc gccgtcgcaa
      421 attgtcgcgg cgattaaatc tcgcgccgat caactgggtg ccagcgtggt ggtgtcgatg
      481 gtagaacgaa gcggcgtcga agcctgtaaa gcggcggtgc acaatcttct cgcgcaacgc
      541 gtcagtgggc tgatcattaa ctatccgctg gatgaccagg atgccattgc tgtggaagct
      601 gcctgcacta atgttccggc gttatttctt gatgtctctg accagacacc catcaacagt
      661 attattttct cccatgagga cggtacgcga ctgggcgtgg agcatctggt cgcattgggt
      721 caccagcaaa tcgcgctgtt agcgggccca ttaagttctg tctcggcgcg tctgcgtctg
      781 gctggctggc ataaatatct cactcgcaat caaattcagc cgatagcgga acgggaaggc
      841 gactggagtg ccatgtccgg ttttcaacaa accatgcaaa tgctgaatga gggcatcgtt
      901 c
//
