LOCUS       sequence-147590-         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MCP-3XBFPnls.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Ma H, Tu LC, Naseri A, Huisman M, Zhang S, Grunwald D, Pederson T
  TITLE     Multiplexed labeling of genomic loci with dCas9 and engineered 
            sgRNAs using CRISPRainbow.
  JOURNAL   Nat Biotechnol. 2016 Apr 18. doi: 10.1038/nbt.3526.
  PUBMED    27088723
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Apr 18. doi: 10.1038/nbt.3526."
COMMENT     Sequence Label: pHAGE-TO-MCP-3XBFPnls
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(55..75)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(82..792)
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
ORIGIN
        1 ataccagtca atctttcaca aattttgtaa tccagaggtt gattatctag attacacttt
       61 ccgctttttc tttggctcga gattaagctt gtgccccagt ttgctaggga ggtcgcagta
      121 tctggccact gccacctcgt gctgctcgac gtaggtttcg ttgttggcct ccttgattct
      181 ttccagtctg tagtccacat agtagacgcc aggcatcttg aggttcttag cgggtttctt
      241 ggatctatat gtggtcttgg cgtttgcgat cagatggctc ccgcccacga gcttcagggc
      301 catgtcgttt ctgccttcca ggccgccgtc agcggggtac agcgtctcgg tgaaggcctc
      361 ccagccgagt gttttcttct gcatcacagg gccgttggat gtgaagttca cccctctgat
      421 cttgacgttg tagatgaggc agccgtcctg gaggctggtg tcctgggtag cggtcagcac
      481 gcccccgtct tcgtatgtgg tgactctctc ccatgtgaag ccctcaggga aggactgctt
      541 gaagaagtcg gggatgccct gggtgtggtt gatgaaggtc ttgctgccgt agaggaagct
      601 agtagccagg atgtcgaagg cgaaggggag agggccgccc tcgaccacct tgattctcat
      661 ggtctgggtg ccctcgtagg gcttgccttc gccctcggat gtgcacttga agtgatggtt
      721 gtccacggtg ccctccatgt acagcttcat gtgcatgttc tccttaatca gctcttcgcc
      781 cttagacacc atgctaccat cgatgctacc attaagcttg tgccccagtt tgctagggag
      841 gtcgcagtat ctggccactg ccacctcgtg ctgctcgacg
//
