LOCUS       sequence-147592-         752 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  reprogrammed sortase A variant that recognizes alternative substrate
            LPXSG with high activity and specificity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Dorr BM, Ham HO, An C, Chaikof EL, Liu DR
  TITLE     Reprogramming the specificity of sortase enzymes.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Sep 16;111(37):13343-8. doi: 
            10.1073/pnas.1411179111. Epub 2014 Sep 3.
  PUBMED    25187567
REFERENCE   2  (bases 1 to 752)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1411179111"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2014-09-16- 16"; volume: "111"; issue: "37"; pages: "13343-8"
COMMENT     Sequence Label: eSrtA(4S-9) pET29b
FEATURES             Location/Qualifiers
     source          1..752
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             473..490
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(543..561)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      557..604
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(728..747)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 actttaagaa ggagatatac atatgcaggc gaaaccgcag attccgaaag ataaaagcaa
       61 agtggcgggc tatattgaaa ttccggatgc ggatattaaa gaaccggtgt atccgggccc
      121 ggcgacccgt gaacagctgg atcgtggtgt gtgctttgtg gaagaaaacg aaagcctgga
      181 tgatcagaac attagcatta ccggccatac cgcgattgac cgtccgaact atcagtttac
      241 caacctgagg gcggcgaaaa aaggcagcat ggtgtatctt aaagtgggca acgaaacccg
      301 taaatataaa atgaccagca ttcgtaacgt gaaaccgacc gcggtggaag tgctggatga
      361 acagaaaggc aaagataaac agctgaccct ggtgacctgc gatgattata actttgaaac
      421 cggcgtgtgg gaaacccgta aaatttttgt ggcgaccgaa gtgaaaggat cccaccacca
      481 ccaccaccac tgagatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc
      541 caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt
      601 tttgctgaaa ggaggaacta tatccggatt ggcgaatggg acgcgccctg tagcggcgca
      661 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
      721 gcgcccgctc ctttcgcttt cttcccttcc tt
//
