LOCUS       sequence-147597-         858 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plant expression of Cas9 and gRNA empty backbone; Hygro Resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Kim H, Kim ST, Ryu J, Choi MK, Kweon J, Kang BC, Ahn HM, Bae S, Kim 
            JS, Kim SG
  TITLE     A simple, flexible and high-throughput cloning system for plant 
            genome editing via CRISPR-Cas system.
  JOURNAL   J Integr Plant Biol. 2016 Mar 4. doi: 10.1111/jipb.12474.
  PUBMED    26946469
REFERENCE   2  (bases 1 to 858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Integr 
            Plant Biol. 2016 Mar 4. doi: 10.1111/jipb.12474."
COMMENT     Sequence Label: pHAtC
FEATURES             Location/Qualifiers
     source          1..858
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ggcaatggaa tccgaggagg tttcccgata ttaccctttg ttgaaaagtc tcaatagccc
       61 tttggtcttc tgagactgta tctttgatat tcttggagta gacgagagtg tcgtgctcca
      121 ccatgttgac gaagattttc ttcttgtcat tgagtcgtaa aagactctgt atgaactgtt
      181 cgccagtctt cacggcgagt tctgttagat cctcgatctg aatttttgac tccatggcct
      241 ttgattcagt aggaactact ttcttagaga ctccaatctc tattacttgc cttggtttat
      301 gaagcaagcc ttgaatcgtc catactggaa tagtacttct gatcttgaga aatatatctt
      361 tctctgtgtt cttgatgcag ttagtcctga atcttttgac tgcatcttta accttcttgg
      421 gaaggtattt gatctcctgg agattattac tcgggtagat cgtcttgatg agacctgccg
      481 cgtaggcctc tctaaccatc tgtgggtcag cattctttct gaaattgaag aggctaatct
      541 tctcattatc ggtggtgaac atggtatcgt caccttctcc gtcgaacttt cttcctagat
      601 cgtagagata gagaaagtcg tccatggtga tctccggggc aaaggagatc agcttggctc
      661 tagtcgacca tatgggagag ctcgaattcg aatgattagg catcgaacct tcaagaattt
      721 gattgaataa aacatcttca ttcttaagat atgaagataa tcttcaaaag gcccctggga
      781 atctgaaaga agagaagcag gcccatttat atgggaaaga acaatagtat ttcttatata
      841 ggcccattta agttgaaa
//
