LOCUS       sequence-147598-         910 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plant expression of Cas9 and gRNA empty backbone; Hygro Resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Kim H, Kim ST, Ryu J, Choi MK, Kweon J, Kang BC, Ahn HM, Bae S, Kim 
            JS, Kim SG
  TITLE     A simple, flexible and high-throughput cloning system for plant 
            genome editing via CRISPR-Cas system.
  JOURNAL   J Integr Plant Biol. 2016 Mar 4. doi: 10.1111/jipb.12474.
  PUBMED    26946469
REFERENCE   2  (bases 1 to 910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Integr 
            Plant Biol. 2016 Mar 4. doi: 10.1111/jipb.12474."
COMMENT     Sequence Label: pHAtC
FEATURES             Location/Qualifiers
     source          1..910
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tgaatcactg cgaccggccc tcccgcgacc cagccgagcg agcttagcga actgtggacg
       61 agaactgtgc caccaagcgt aaggccgttc tctcgcattt gccttgctag gctcgcgcga
      121 gttgctggct gaggcgttct cgaaatcagc tcttgttcgg tcggcatcta ctctattcct
      181 ttgccctcgg acgagtgctg gggcgtcggt ttccactatc ggcgagtact tctacacagc
      241 catcggtcca gacggccgcg cttctgcggg cgatttgtgt acgcccgaca gtcccggctc
      301 cggatcggac gattgcgtcg catcgaccct gcgcccaagc tgcatcatcg aaattgccgt
      361 caaccaagct ctgatagagt tggtcaagac caatgcggag catatacgcc cggagccttg
      421 gcgatcctgc aagctccgga tgcctccgct cgaagtagcg cgtctgctgc tccatacaag
      481 ccaaccacgg cctccagaag aagatgttgg cgacctcgta ttgggaatcc ccgaacatcg
      541 cctcgctcca gtcaatgacc gctgttatgc ggccattgtc cgtcaggaca ttgttggagc
      601 cgaaatccgc gtgcacgagg tgccggactt cggggcagtc ctcggcccaa agcatcagct
      661 catcgagagc ctgcgcgacg gacgcactga cggtgtcgtc catcacagtt tgccagtgat
      721 acacatgggg atcagcaatc gcgcatatga aatcacgcca tgtagtgtat tgaccgattc
      781 cttgcggtcc gaatgggccg aacccgctcg tctggctaag atcggccgca gcgatagcat
      841 ccatagcctc cgcgaccggc tgaagaacag cgggcagttc ggtttcaggc aggtcttgca
      901 acgtgacacc
//
