LOCUS       sequence-147599-         935 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plant expression of Cas9 and gRNA empty backbone; Basta resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Kim H, Kim ST, Ryu J, Choi MK, Kweon J, Kang BC, Ahn HM, Bae S, Kim 
            JS, Kim SG
  TITLE     A simple, flexible and high-throughput cloning system for plant 
            genome editing via CRISPR-Cas system.
  JOURNAL   J Integr Plant Biol. 2016 Mar 4. doi: 10.1111/jipb.12474.
  PUBMED    26946469
REFERENCE   2  (bases 1 to 935)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Integr 
            Plant Biol. 2016 Mar 4. doi: 10.1111/jipb.12474."
COMMENT     Sequence Label: pBAtC
FEATURES             Location/Qualifiers
     source          1..935
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(569..748)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_RNA        complement(852..927)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 ggacggggcg gcaccggcag gctgaagtcc agctgccaga aacccacgtc atgccagttc
       61 ccgtgcttga agccggccgc ccgcagcatg ccgcgggggg catatccgag cgcctcgtgc
      121 atgcgcacgc tcgggtcgtt gggcagcccg atgacagcga ccacgctctt gaagccctgt
      181 gcctccaggg acttcagaag gtgggtgtag agcgtggagc ccagtcccgt ccgctggtgg
      241 cggggggaga cgtacacggt cgactcggcc gtccagtcgt aggcgttgcg tgccttccag
      301 ggacccgcgt aggcgatgcc ggcgacctcg ccgtccacct cggcgacgag ccagggatag
      361 cgctcccgca gacggacgag gtcgtccgtc cactcctgcg gttcctgcgg ctcggtacgg
      421 aagttgaccg tgcttgtctc gatgtagtgg ttgacgatgg tgcagaccgc cggcatgtcc
      481 gcctcggtgg cacggcggat gtcggccggg cgtcgttctg ggctcatggt agatcccctc
      541 gatcgagttg agagtgaata tgagactcta attggatacc gaggggaatt tatggaacgt
      601 cagtggagca tttttgacaa gaaatatttg ctagctgata gtgaccttag gcgacttttg
      661 aacgcgcaat aatggtttct gacgtatgtg cttagctcat taaactccag aaacccgcgg
      721 ctcagtggct ccttcaacgt tgcggttctg tcagttccaa acgtaaaacg gcttgtcccg
      781 cgtcatcggc gggggtcata acgtgactcc cttaattctc cgctcatgat cgataattcg
      841 agctcaaaaa agcaccgact cggtgccact ttttcaagtt gataacggac tagccttatt
      901 ttaacttgct atttctagct ctaaaacctc tgcag
//
