LOCUS       sequence-147600-         568 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  sgKEAP1-1, puromycin selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 568)
  AUTHORS   Krall EB, Wang B, Munoz DM, Ilic N, Raghavan S, Niederst MJ, Yu K, 
            Ruddy DA, Aguirre AJ, Kim JW, Redig AJ, Gainor JF, Williams JA, 
            Asara JM, Doench JG, Janne PA, Shaw AT, McDonald Iii RE, Engelman 
            JA, Stegmeier F, Schlabach MR, Hahn WC
  TITLE     KEAP1 loss modulates sensitivity to kinase targeted therapy in lung 
            cancer.
  JOURNAL   Elife. 2017 Feb 1;6:e18970. doi: 10.7554/eLife.18970.
  PUBMED    28145866
REFERENCE   2  (bases 1 to 568)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2017-02-1"; volume: "6:e18970. doi"; pages: " 
            10.7554/eLife.18970"
COMMENT     Sequence Label: pXPR_BRD003 sgKEAP1-1
FEATURES             Location/Qualifiers
     source          1..568
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     120..139
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        219..294
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    350..467
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ttagagagat aattagaatt aatttgactg taaacacaaa gatattagta caaaatacgt
       61 gacgtagaaa gtaataattt cttgggtagt ttgcagtttt aaaattatgt tttaaaatgg
      121 actatcatat gcttaccgta acttgaaagt atttcgattt cttggcttta tatatcttgt
      181 ggaaaggacg aaacaccgct tgtgggccat gaactggggt tttagagcta gaaatagcaa
      241 gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt
      301 aagcttggcg taactagatc ttgagacaaa tggcagtatt catccacaat tttaaaagaa
      361 aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca
      421 tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca
      481 gggacagcag agatccactt tggcgccggc tcgagggggc ccgggtgcaa agatggataa
      541 agttttaaac agagaggaat ctttgcag
//
