LOCUS       sequence-147606-         701 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  sgKEAP1-2, puromycin selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 701)
  AUTHORS   Krall EB, Wang B, Munoz DM, Ilic N, Raghavan S, Niederst MJ, Yu K, 
            Ruddy DA, Aguirre AJ, Kim JW, Redig AJ, Gainor JF, Williams JA, 
            Asara JM, Doench JG, Janne PA, Shaw AT, McDonald Iii RE, Engelman 
            JA, Stegmeier F, Schlabach MR, Hahn WC
  TITLE     KEAP1 loss modulates sensitivity to kinase targeted therapy in lung 
            cancer.
  JOURNAL   Elife. 2017 Feb 1;6:e18970. doi: 10.7554/eLife.18970.
  PUBMED    28145866
REFERENCE   2  (bases 1 to 701)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2017-02-1"; volume: "6:e18970. doi"; pages: " 
            10.7554/eLife.18970"
COMMENT     Sequence Label: pXPR_BRD003 sgKEAP1-2
FEATURES             Location/Qualifiers
     source          1..701
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     121..140
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        220..295
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    351..468
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gttagagaga taattagaat taatttgact gtaaacacaa agatattagt acaaaatacg
       61 tgacgtagaa agtaataatt tcttgggtag tttgcagttt taaaattatg ttttaaaatg
      121 gactatcata tgcttaccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg
      181 tggaaaggac gaaacaccgt gtgtcctcca cgtcatgaag ttttagagct agaaatagca
      241 agttaaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt
      301 taagcttggc gtaactagat cttgagacaa atggcagtat tcatccacaa ttttaaaaga
      361 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
      421 atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac
      481 agggacagca gagatccact ttggcgccgg ctcgaggggg cccgggtgca aagatggata
      541 aagttttaaa cagagaggaa tctttgcagc taatggacct tctaggtctt gaaaggagtg
      601 ggaattggct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag
      661 ttggggggag gggtcggcaa ttgatccggt gcctagagaa g
//
