LOCUS       sequence-147610-         783 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 + sgATXN1L-2 with blasticidin selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Wang B, Krall EB, Aguirre AJ, Kim M, Widlund HR, Doshi MB, Sicinska 
            E, Sulahian R, Goodale A, Cowley GS, Piccioni F, Doench JG, Root DE,
            Hahn WC
  TITLE     ATXN1L, CIC, and ETS Transcription Factors Modulate Sensitivity to 
            MAPK Pathway Inhibition.
  JOURNAL   Cell Rep. 2017 Feb 7;18(6):1543-1557. doi: 
            10.1016/j.celrep.2017.01.031.
  PUBMED    28178529
REFERENCE   2  (bases 1 to 783)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.01"; journalName: "Cell Rep"; date: 
            "2017-02-7- 7"; volume: "18"; issue: "6"; pages: "1543-1557"
COMMENT     Sequence Label: PXPR007 sgATXN1L-2
FEATURES             Location/Qualifiers
     source          1..783
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    92..209
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_RNA        complement(257..332)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(354..602)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(412..431)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(582..602)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 tgtgggcgat gtgcgctctg cccactgacg ggcaccggag ccactcgagc cggcgccaaa
       61 gtggatctct gctgtccctg taataaaccc gaaaattttg aatttttgta atttgttttt
      121 gtaattcttt agtttgtatg tctgttgcta ttatgtctac tattctttcc cctgcactgt
      181 accccccaat cccccctttt cttttaaaat tgtggatgaa tactgccatt tgtctcaaga
      241 tctagaattc aaaaaagcac cgactcggtg ccactttttc aagttgataa cggactagcc
      301 ttattttaac ttgctatttc tagctctaaa accgctgcag ttcattgggt ctcggtgttt
      361 cgtcctttcc acaagatata taaagccaag aaatcgaaat actttcaagt tacggtaagc
      421 atatgatagt ccattttaaa acataatttt aaaactgcaa actacccaag aaattattac
      481 tttctacgtc acgtattttg tactaatatc tttgtgttta cagtcaaatt aattctaatt
      541 atctctctaa cagccttgta tcgtatatgc aaatatgaag gaatcatggg aaataggccc
      601 tctctgggtc ccctcggggt tgggaggtgg gtctgaaacg ataatggtga atatccctgc
      661 ctaactctat tcactataga aagtacagca aaaactattc ttaaacctac caagcctcct
      721 actatcatta tgaataattt tatataccac agccaatttg ttatgttaaa ccaattccac
      781 aaa
//
