LOCUS       sequence-147618-         781 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 + sgATXN1L-1 with blasticidin selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Wang B, Krall EB, Aguirre AJ, Kim M, Widlund HR, Doshi MB, Sicinska 
            E, Sulahian R, Goodale A, Cowley GS, Piccioni F, Doench JG, Root DE,
            Hahn WC
  TITLE     ATXN1L, CIC, and ETS Transcription Factors Modulate Sensitivity to 
            MAPK Pathway Inhibition.
  JOURNAL   Cell Rep. 2017 Feb 7;18(6):1543-1557. doi: 
            10.1016/j.celrep.2017.01.031.
  PUBMED    28178529
REFERENCE   2  (bases 1 to 781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.01"; journalName: "Cell Rep"; date: 
            "2017-02-7- 7"; volume: "18"; issue: "6"; pages: "1543-1557"
COMMENT     Sequence Label: PXPR007 sgATXN1L-1
FEATURES             Location/Qualifiers
     source          1..781
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    89..206
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_RNA        complement(254..329)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(351..599)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(409..428)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(579..599)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 gggcgatgtg cgctctgccc actgacgggc accggagcca ctcgagccgg cgccaaagtg
       61 gatctctgct gtccctgtaa taaacccgaa aattttgaat ttttgtaatt tgtttttgta
      121 attctttagt ttgtatgtct gttgctatta tgtctactat tctttcccct gcactgtacc
      181 ccccaatccc cccttttctt ttaaaattgt ggatgaatac tgccatttgt ctcaagatct
      241 agaattcaaa aaagcaccga ctcggtgcca ctttttcaag ttgataacgg actagcctta
      301 ttttaacttg ctatttctag ctctaaaacc ggtgtgctgg tgctgctacc ggtgtttcgt
      361 cctttccaca agatatataa agccaagaaa tcgaaatact ttcaagttac ggtaagcata
      421 tgatagtcca ttttaaaaca taattttaaa actgcaaact acccaagaaa ttattacttt
      481 ctacgtcacg tattttgtac taatatcttt gtgtttacag tcaaattaat tctaattatc
      541 tctctaacag ccttgtatcg tatatgcaaa tatgaaggaa tcatgggaaa taggccctct
      601 ctgggtcccc tcggggttgg gaggtgggtc tgaaacgata atggtgaata tccctgccta
      661 actctattca ctatagaaag tacagcaaaa actattctta aacctaccaa gcctcctact
      721 atcattatga ataattttat ataccacagc caatttgtta tgttaaacca attccacaaa
      781 c
//
