LOCUS       sequence-147628-         846 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Van Engelenburg SB, Palmer AE
  TITLE     Imaging type-III secretion reveals dynamics and spatial segregation 
            of Salmonella effectors.
  JOURNAL   Nat Methods. 2010 Apr;7(4):325-30. doi: 10.1038/nmeth.1437. Epub 
            2010 Mar 14.
  PUBMED    20228815
REFERENCE   2  (bases 1 to 846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1437"; journalName: "Nat Methods"; date: "2010-04"; 
            volume: "7"; issue: "4"; pages: "325-30"
COMMENT     Sequence Label: pLAMP1-mCherry
FEATURES             Location/Qualifiers
     source          1..846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    77..101
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      103..112
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agatcaacaa gtttgtacaa aaaagcaggc taccatggcg gcccccggca
      121 gcgcccggcg acccctgctg ctgctactgc tgttgctgct gctcggcctc atgcattgtg
      181 cgtcagcagc aatgtttatg gtgaaaaatg gcaacgggac cgcgtgcata atggccaact
      241 tctctgctgc cttctcagtg aactacgaca ccaagagtgg ccctaagaac atgacctttg
      301 acctgccatc agatgccaca gtggtgctca accgcagctc ctgtggaaaa gagaacactt
      361 ctgaccccag tctcgtgatt gcttttggaa gaggacatac actcactctc aatttcacga
      421 gaaatgcaac acgttacagc gtccagctca tgagttttgt ttataacttg tcagacacac
      481 accttttccc caatgcgagc tccaaagaaa tcaagactgt ggaatctata actgacatca
      541 gggcagatat agataaaaaa tacagatgtg ttagtggcac ccaggtccac atgaacaacg
      601 tgaccgtaac gctccatgat gccaccatcc aggcgtacct ttccaacagc agcttcagca
      661 ggggagagac acgctgtgaa caagacaggc cttccccaac cacagcgccc cctgcgccac
      721 ccagcccctc gccctcaccc gtgcccaaga gcccctctgt ggacaagtac aacgtgagcg
      781 gcaccaacgg gacctgcctg ctggccagca tggggctgca gctgaacctc acctatgaga
      841 ggaagg
//
