LOCUS       sequence-147629-       10107 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression of TRI-2A (hHO1/F2A/hCD73/F2A/hCD39).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10107)
  AUTHORS   Cinti A, De Giorgi M, Chisci E, Arena C, Galimberti G, Farina L, 
            Bugarin C, Rivolta I, Gaipa G, Smolenski RT, Cerrito MG, Lavitrano 
            M, Giovannoni R
  TITLE     Simultaneous Overexpression of Functional Human HO-1, E5NT and 
            ENTPD1 Protects Murine Fibroblasts against TNF-alpha-Induced Injury 
            In Vitro.
  JOURNAL   PLoS One. 2015 Oct 29;10(10):e0141933. doi: 
            10.1371/journal.pone.0141933. eCollection 2015.
  PUBMED    26513260
REFERENCE   2  (bases 1 to 10107)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-10-29"; volume: "10(10):e0141933. doi"; pages: " 
            10.1371/journal.pone.0141933. eCollection 2015"
COMMENT     Sequence Label: pCX-TRI-2A
FEATURES             Location/Qualifiers
     source          1..10107
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        15..394
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        397..672
                     /label=chicken beta-actin promoter
     intron          673..1681
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1605..1627
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     CDS             2614..2679
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     CDS             4423..4488
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     primer_bind     complement(6120..6139)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6185..6240
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6239..6258)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6599..6615)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6599..6615)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6612..6634)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6623..6639
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6647..6653,6654..6671,6672..6677))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6692..6713
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     polyA_signal    6895..7016
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6932..6951)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6986..7005
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(7023..7478)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7160..7181)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7372..7391
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        7505..7609
                     /gene="bla"
                     /label=AmpR promoter
     promoter        7611..7968
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7611..7802
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7634..7654)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7819..7954
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8003..8797
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             8003..8797
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8057..8076)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8667..8686
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(8985..9004)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    9029..9076
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      9405..9993
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9894..9913
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattat ggtcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc
       61 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
      121 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
      181 aacgccaata gggactttcc attgacgtca atgggtggac tatttacggt aaactgccca
      241 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
      301 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
      361 gtacatctac gtattagtca tcgctattac catgggtcga ggtgagcccc acgttctgct
      421 tcactctccc catctccccc ccctccccac ccccaatttt gtatttattt attttttaat
      481 tattttgtgc agcgatgggg gcgggggggg ggggggcgcg cgccaggcgg ggcggggcgg
      541 ggcgaggggc ggggcggggc gaggcggaga ggtgcggcgg cagccaatca gagcggcgcg
      601 ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc
      661 gcgcggcggg cgggagtcgc tgcgttgcct tcgccccgtg ccccgctccg cgccgcctcg
      721 cgccgcccgc cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc
      781 ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac ggctcgtttc ttttctgtgg
      841 ctgcgtgaaa gccttaaagg gctccgggag ggccctttgt gcggggggga gcggctcggg
      901 gggtgcgtgc gtgtgtgtgt gcgtggggag cgccgcgtgc ggcccgcgct gcccggcggc
      961 tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc gcgtgtgcgc gaggggagcg
     1021 cggccggggg cggtgccccg cggtgcgggg gggctgcgag gggaacaaag gctgcgtgcg
     1081 gggtgtgtgc gtgggggggt gagcaggggg tgtgggcgcg gcggtcgggc tgtaaccccc
     1141 ccctgcaccc ccctccccga gttgctgagc acggcccggc ttcgggtgcg gggctccgtg
     1201 cggggcgtgg cgcggggctc gccgtgccgg gcggggggtg gcggcaggtg ggggtgccgg
     1261 gcggggcggg gccgcctcgg gccggggagg gctcggggga ggggcgcggc ggccccggag
     1321 cgccggcggc tgtcgaggcg cggcgagccg cagccattgc cttttatggt aatcgtgcga
     1381 gagggcgcag ggacttcctt tgtcccaaat ctggcggagc cgaaatctgg gaggcgccgc
     1441 cgcaccccct ctagcgggcg cgggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg
     1501 gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccatctccag cctcggggct
     1561 gccgcagggg gacggctgcc ttcggggggg acggggcagg gcggggttcg gcttctggcg
     1621 tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct ttttcctaca
     1681 gctcctgggc aacgtgctgg ttgttgtgct gtctcatcat tttggcaaag aattcccacc
     1741 atggagcgtc cgcaacccga cagcatgccc caggatttgt cagaggccct gaaggaggcc
     1801 accaaggagg tgcacaccca ggcagagaat gctgagttca tgaggaactt tcagaagggc
     1861 caggtgaccc gagacggctt caagctggtg atggcctccc tgtaccacat ctatgtggcc
     1921 ctggaggagg agattgagcg caacaaggag agcccagtct tcgcccctgt ctacttccca
     1981 gaagagctgc accgcaaggc tgccctggag caggacctgg ccttctggta cgggccccgc
     2041 tggcaggagg tcatccccta cacaccagcc atgcagcgct atgtgaagcg gctccacgag
     2101 gtggggcgca cagagcccga gctgctggtg gcccacgcct acacccgcta cctgggtgac
     2161 ctgtctgggg gccaggtgct caaaaagatt gcccagaaag ccctggacct gcccagctct
     2221 ggcgagggcc tggccttctt caccttcccc aacattgcca gtgccaccaa gttcaagcag
     2281 ctctaccgct cccgcatgaa ctccctggag atgactcccg cagtcaggca gagggtgata
     2341 gaagaggcca agactgcgtt cctgctcaac atccagctct ttgaggagtt gcaggagctg
     2401 ctgacccatg acaccaagga ccagagcccc tcacgggcac cagggcttcg ccagcgggcc
     2461 agcaacaaag tgcaagattc tgcccccgtg gagactccca gagggaagcc cccactcaac
     2521 acccgctccc aggctccgct tctccgatgg gtccttacac tcagctttct ggtggcgaca
     2581 gttgctgtag ggctttatgc catgtgagct agcgtgaaac agactttgaa ttttgacctt
     2641 ctcaagttgg cgggagacgt ggagtccaac ccagggcccg gcagcggcct taagatgtgt
     2701 ccccgagccg cgcgggcgcc cgcgacgcta ctcctcgccc tgggcgcggt gctgtggcct
     2761 gcggctggcg cctgggagct tacgattttg cacaccaacg acgtgcacag ccggctggag
     2821 cagaccagcg aggactccag caagtgcgtc aacgccagcc gctgcatggg tggcgtggct
     2881 cggctcttca ccaaggttca gcagatccgc cgcgccgaac ccaacgtgct gctgctggac
     2941 gccggcgacc agtaccaggg cactatctgg ttcaccgtgt acaagggcgc cgaggtggcg
     3001 cacttcatga acgccctgcg ctacgatgcc atggcactgg gaaatcatga atttgataat
     3061 ggtgtggaag gactgatcga gccactcctc aaagaggcca aatttccaat tctgagtgca
     3121 aacattaaag caaaggggcc actagcatct caaatatcag gactttattt gccatataaa
     3181 gttcttcctg ttggtgatga agttgtggga atcgttggat acacttccaa agaaacccct
     3241 tttctctcaa atccagggac aaatttagtg tttgaagatg aaatcactgc attacaacct
     3301 gaagtagata agttaaaaac tctaaatgtg aacaaaatta ttgcactggg acattcgggt
     3361 tttgaaatgg ataaactcat cgctcagaaa gtgaggggtg tggacgtcgt ggtgggagga
     3421 cactccaaca catttcttta cacaggcaat ccaccttcca aagaggtgcc tgctgggaag
     3481 tacccattca tagtcacttc tgatgatggg cggaaggttc ctgtagtcca ggcctatgct
     3541 tttggcaaat acctaggcta tctgaagatc gagtttgatg aaagaggaaa cgtcatctct
     3601 tcccatggaa atcccattct tctaaacagc agcattcctg aagatccaag cataaaagca
     3661 gacattaaca aatggaggat aaaattggat aattattcta cccaggaatt agggaaaaca
     3721 attgtctatc tggatggctc ctctcaatca tgccgcttta gagaatgcaa catgggcaac
     3781 ctgatttgtg atgcaatgat taacaacaac ctgagacaca cggatgaaat gttctggaac
     3841 cacgtatcca tgtgcatttt aaatggaggt ggtatccggt cgcccattga tgaacgcaac
     3901 aatggcacaa ttacctggga gaacctggct gctgtattgc cctttggagg cacatttgac
     3961 ctagtccagt taaaaggttc caccctgaag aaggcctttg agcatagcgt gcaccgctac
     4021 ggccagtcca ctggagagtt cctgcaggtg ggcggaatcc atgtggtgta tgatctttcc
     4081 cgaaaacctg gagacagagt agtcaaatta aatgttcttt gcaccaagtg tcgagtgccc
     4141 agttatgacc ctctcaaaat ggacgaggta tataaggtga tcctcccaaa cttcctggcc
     4201 aatggtggag atgggttcca gatgataaaa gatgaattat taagacatga ctctggtgac
     4261 caagatatca acgtggtttc tacatatatc tccaaaatga aagtaattta tccagcagtt
     4321 gaaggtcgga tcaagttttc cacaggaagt cactgccatg gaagcttttc tttaatattt
     4381 ctttcacttt gggcagtgat ctttgtttta taccaaggat ccgtgaaaca gactttgaat
     4441 tttgaccttc tcaagttggc gggagacgtg gagtccaacc cagggcccgg cagcggcctc
     4501 gagatggaag atacaaagga gtctaacgtg aagacatttt gctccaagaa tatcctagcc
     4561 atccttggct tctcctctat catagctgtg atagctttgc ttgctgtggg gttgacccag
     4621 aacaaagcat tgccagaaaa cgttaagtat gggattgtgc tggatgcggg ttcttctcac
     4681 acaagtctat acatctataa gtggccagca gaaaaggaga atgacacagg cgtggtgcat
     4741 caagtagaag aatgcagggt taaaggtcct ggaatctcaa aatttgttca gaaagtaaat
     4801 gaaataggca tttacctgac tgattgcatg gaaagagcta gggaagtgat tccaaggtcc
     4861 cagcaccaag agacacccgt ttacctggga gccacggcag gcatgcggtt gctcaggatg
     4921 gaaagtgaag agttggcaga cagggttctg gatgtggtgg agaggagcct cagcaactac
     4981 ccctttgact tccagggtgc caggatcatt actggccaag aggaaggtgc ctatggctgg
     5041 attactatca actatctgct gggcaaattc agtcagaaaa caaggtggtt cagcatagtc
     5101 ccatatgaaa ccaataatca ggaaaccttt ggagctttgg accttggggg agcctctaca
     5161 caagtcactt ttgtacccca aaaccagact atcgagtccc cagataatgc tctgcaattt
     5221 cgcctctatg gcaaggacta caatgtctac acacatagct tcttgtgcta tgggaaggat
     5281 caggcactct ggcagaaact ggccaaggac attcaggttg caagtaatga aattctcagg
     5341 gacccatgct ttcatcctgg atataagaag gtagtgaacg taagtgacct ttacaagacc
     5401 ccctgcacca agagatttga gatgactctt ccattccagc agtttgaaat ccagggtatt
     5461 ggaaactatc aacaatgcca tcaaagcatc ctggagctct tcaacaccag ttactgccct
     5521 tactcccagt gtgccttcaa tgggattttc ttgccaccac tccaggggga ttttggggca
     5581 ttttcagctt tttactttgt gatgaagttt ttaaacttga catcagagaa agtctctcag
     5641 gaaaaggtga ctgagatgat gaaaaagttc tgtgctcagc cttgggagga gataaaaaca
     5701 tcttacgctg gagtaaagga gaagtacctg agtgaatact gcttttctgg tacctacatt
     5761 ctctccctcc ttctgcaagg ctatcatttc acagctgatt cctgggagca catccatttc
     5821 attggcaaga tccagggcag cgacgccggc tggactttgg gctacatgct gaacctgacc
     5881 aacatgatcc cagctgagca accattgtcc acacctctct cccactccac ctatgtcttc
     5941 ctcatggttc tattctccct ggtccttttc acagtggcca tcataggctt gcttatcttt
     6001 cacaagcctt catatttctg gaaagatatg gtataggaat tcactcctca ggtgcaggct
     6061 gcctatcaga aggtggtggc tggtgtggcc aatgccctgg ctcacaaata ccactgagat
     6121 ctttttccct ctgccaaaaa ttatggggac atcatgaagc cccttgagca tctgacttct
     6181 ggctaataaa ggaaatttat tttcattgca atagtgtgtt ggaatttttt gtgtctctca
     6241 ctcggaagga catatgggag ggcaaatcat ttaaaacatc agaatgagta tttggtttag
     6301 agtttggcaa catatgccat atgctggctg ccatgaacaa aggtggctat aaagaggtca
     6361 tcagtatatg aaacagcccc ctgctgtcca ttccttattc catagaaaag ccttgacttg
     6421 aggttagatt ttttttatat tttgttttgt gttatttttt tctttaacat ccctaaaatt
     6481 ttccttacat gttttactag ccagattttt cctcctctcc tgactactcc cagtcatagc
     6541 tgtccctctt ctcttatgaa gatccctcga cctgcagccc aagcttggcg taatcatggt
     6601 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     6661 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     6721 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagcggatc caccggatct
     6781 agataactga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac
     6841 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
     6901 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     6961 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttaacgc
     7021 gtaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
     7081 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
     7141 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
     7201 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
     7261 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
     7321 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
     7381 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
     7441 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa
     7501 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     7561 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagtc ctgaggcgga
     7621 aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
     7681 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     7741 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     7801 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     7861 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     7921 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaga tcgatcaaga
     7981 gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc
     8041 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga
     8101 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct
     8161 gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac
     8221 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct
     8281 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt
     8341 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt
     8401 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt
     8461 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag
     8521 gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt
     8581 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg
     8641 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg
     8701 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg
     8761 catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg
     8821 accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat
     8881 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
     8941 gatctcatgc tggagttctt cgcccaccct agggggaggc taactgaaac acggaaggag
     9001 acaataccgg aaggaacccg cgctatgacg gcaataaaaa gacagaataa aacgcacggt
     9061 gttgggtcgt ttgttcataa acgcggggtt cggtcccagg gctggcactc tgtcgatacc
     9121 ccaccgagac cccattgggg ccaatacgcc cgcgtttctt ccttttcccc accccacccc
     9181 ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg gggcggcagg ccctgccata
     9241 gcctcaggtt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     9301 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     9361 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     9421 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     9481 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     9541 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     9601 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     9661 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     9721 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     9781 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     9841 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     9901 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     9961 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
    10021 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
    10081 gtggataacc gtattaccgc catgcat
//
