LOCUS       sequence-147630-         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Citrine reporter for transfer fxns.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Niederholtmeyer H, Sun ZZ, Hori Y, Yeung E, Verpoorte A, Murray RM, 
            Maerkl SJ
  TITLE     Rapid cell-free forward engineering of novel genetic ring 
            oscillators.
  JOURNAL   Elife. 2015 Oct 2;4. doi: 10.7554/eLife.09771.
  PUBMED    26430766
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-10-2"; volume: "4. doi"; pages: " 10.7554/eLife.09771"
COMMENT     Sequence Label: pLacI-BCD7-Citrine P42U18C68T11 v40-1 (536)
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    252..268
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 tcatcctgtc tcttgatcag atcatgatcc cctgcgccat cagatccttg gcggcaagaa
       61 agccatccag tttactttgc agggcttccc aaccttacca gagggcgccc cagctggcaa
      121 ttccgacgtc ggtgcctaat gagtgagcta acttacatta attgcgttgc gctcactgcc
      181 cgctttccag tcgggaaacc tgaagacaac cacgcattgc tgttcataaa tgtgagcgga
      241 taacattgac attgtgagcg gataacaaga tactgagcac agcaagcggg cccaagttca
      301 cttaaaaagg agatcaacaa tgaaagcaat tttcgtactg aaacatctta atcatgctgg
      361 ggagggtttc taatgtctaa aggtgaagaa ttattcactg gtgttgtccc aattttggtt
      421 gaattagatg gtgatgttaa tggtcacaaa ttttctgtct ccggtgaagg tgaaggtgat
      481 gctacttacg gtaaattgac cttaaaattt atttgtacta ctggtaaatt gccagttcca
      541 tggccaacct tagtcactac tttaggttat ggtttgatgt gttttgctag atacccagat
      601 catatgaaac aacatgactt tttcaagtct gccatgccag aaggttatgt tcaagaaaga
      661 actatttttt tcaaagatga cggtaactac aagaccagag ctgaagtcaa gtttgaaggt
      721 gataccttag ttaatagaat cgaattaaaa ggtattgatt ttaaagaaga tggtaacatt
      781 ttaggtcaca aattggaata caactataac tctcacaatg tttacatcat ggctgacaaa
//
