LOCUS       sequence-147645-         614 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Generates truncated version of MAK10 gene, which is required for 
            dsRNA L-A propagation in S.cerevisiae.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 614)
  TITLE     McCusker_pUG-amdSYM-mak10TR
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 614)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pUG-amdSYM-mak10TR
FEATURES             Location/Qualifiers
     source          1..614
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    17..50
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 gtcgacaacc cttaatataa cttcgtataa tgtatgctat acgaagttat taggtctaga
       61 gatctgttta gcttgcctcg tccccgccgg gtcacccggc cagcgacatg gaggcccaga
      121 ataccctcct tgacagtctt gacgtgcgca gctcaggggc atgatgtgac tgtcgcccgt
      181 acatttagcc catacatccc catgtataat catttgcatc catacatttt gatggccgca
      241 cggcgcgaag caaaaattac ggctcctcgc tgcagacctg cgagcaggga aacgctcccc
      301 tcacagacgc gttgaattgt ccccacgccg cgcccctgta gagaaatata aaaggttagg
      361 atttgccact gaggttcttc tttcatatac ttccttttaa aatcttgcta ggatacagtt
      421 ctcacatcac atccgaacat aaacaaggat ccatgccaca atcttgggaa gaattggctg
      481 ctgacaagag agctagattg gctaagacta tcccagacga atggaaggtt caaactttgc
      541 cagctgaaga ctctgttatc gacttcccaa agaagtctgg tatcttgtct gaagctgaat
      601 tgaagatcac tgaa
//
