LOCUS       sequence-147646-         427 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TetR/PhlF/SrpR/LambdaCI/LacI BCD7 5-node v41-2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 427)
  AUTHORS   Niederholtmeyer H, Sun ZZ, Hori Y, Yeung E, Verpoorte A, Murray RM, 
            Maerkl SJ
  TITLE     Rapid cell-free forward engineering of novel genetic ring 
            oscillators.
  JOURNAL   Elife. 2015 Oct 2;4. doi: 10.7554/eLife.09771.
  PUBMED    26430766
REFERENCE   2  (bases 1 to 427)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-10-2"; volume: "4. doi"; pages: " 10.7554/eLife.09771"
COMMENT     Sequence Label: 5n1 (560)
FEATURES             Location/Qualifiers
     source          1..427
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    86..102
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 gattaagatg tttcagtacg aaaattgctt tcattgttga tctccttttt aagtgaactt
       61 gggcccgctt gctgtgctca gtatcttgtt atccgctcac aatgtcaatg ttatccgctc
      121 acatttatga aagcaatgcg gttcgactct caaggaaagc aagcaaagaa aaaaggcgac
      181 agattactct gtcgcctttt ttcctgactc ataacagttc atcaagctgc taangcgtag
      241 ttttcgtcgt ttgctgcctg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
      301 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag ggtggttttt
      361 cttttcacca gtgagacggg caacagctga ttgcccttca ccgcctggcc ctgagagagt
      421 tgcagca
//
