LOCUS       sequence-147648-        1130 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Schirmer EC, Homann OR, Kowal AS, Lindquist S
  TITLE     Dominant gain-of-function mutations in Hsp104p reveal crucial roles 
            for the middle region.
  JOURNAL   Mol Biol Cell 2004 May;15(5):2061-72. Epub 2004 Feb 20.
  PUBMED    14978213
REFERENCE   2  (bases 1 to 1130)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell"; date: "2004-05"; volume: "15(5)"; pages: "2061-72. Epub 2004 
            Feb 20"
COMMENT     Sequence Label: pGALSc104(A503V)
FEATURES             Location/Qualifiers
     source          1..1130
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     6..25
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6..24
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 aattgtaata cgactcacta tagggcgaat tggagctctt ttgctcgggt gtcaagttca
       61 attaaacaaa ttccattagt tttattaatt atatatatta tattactgat tcttgttcga
      121 aagtttttaa aaatcacact atattaaatt aatctaggtc atcatcaatt tccatactgt
      181 cctcattatc gtcatcacct aacgtgtcag cccctatagt agcttcgtga tttggtagaa
      241 cttccagaca ttcttcagct tcctcaggaa cattttcatc acgagattta cccttcttca
      301 agacgacatt gacagtttcc ttatccttga tttcattctt taagatcctt agtgccagtt
      361 tgttcaaaat ttcgttttga attaacctgt tcagtggacg tgcacccata tcatcggaat
      421 aaccatattt ggccaagaag tccttggcct cttgagttaa attcaacttg taatgtttat
      481 cattttgctc gaatctctct tcaatttcct tcaaacgaat atccacgatc ttatgaatag
      541 cttttctaga tagcttgttg aaaatgacta tactagaaat tctgttcaaa aattctggtc
      601 tgaaatgttg cctaacagca cccatgacca aattcttggt agattcttgg atctttgatc
      661 cttgttgaga attgataaat tcagcaccta gattggaagt catgatgaca atacaattgg
      721 aacagtcgat cgtcttacct tgaccactag taattctacc gtcatccaac atttgtagca
      781 tgacagtcaa aacatcagga tgtgcctttt ctacttcatc gaataacaaa acggagtatg
      841 gtttgtattg cagttggtta gttaaaaagc caccttcatc gtacccgaca taacctgccg
      901 tggtacccaa caacttagag accgcatact tctcgcttaa ttcagaacaa tcgaccctga
      961 tcatcatgtc ctcatcatta aacaaaaatc cagcaacttt tttagccaat tcagttttac
     1021 cggaaccgga caaacctaaa aataagaagg atgctggttg ccttggatta gctaaacctg
     1081 atctagacaa tctaacggca ttggaaacag ctttaatggc atccatttgg
//
