LOCUS       sequence-147650-         887 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive expression of Cas9, inducible expression of the Red 
            recombineering system, and inducible expression of the plasmid 
            curing system for CRISPR mediated genome editing of E. coli..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Li Y, Lin Z, Huang C, Zhang Y, Wang Z, Tang YJ, Chen T, Zhao X
  TITLE     Metabolic engineering of Escherichia coli using CRISPR-Cas9 
            meditated genome editing.
  JOURNAL   Metab Eng. 2015 Sep;31:13-21. doi: 10.1016/j.ymben.2015.06.006. Epub
            2015 Jun 30.
  PUBMED    26141150
REFERENCE   2  (bases 1 to 887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2015-09"; volume: "31:13-21. doi"; pages: " 
            10.1016/j.ymben.2015.06.006. Epub 2015 Jun 30"
COMMENT     Sequence Label: pREDCas9
FEATURES             Location/Qualifiers
     source          1..887
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     repeat_region   497..532
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     promoter        complement(679..844)
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     complement(737..756)
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
ORIGIN
        1 tccgtttcca cggtgtgcgt cacccggcaa ccttgggcag cagcgaagtc gaggcatttc
       61 tgtcctggct ggcgaacgag cgcaaggttt cggtctccac gcatcgtcag gcattggcgg
      121 ccttgctgtt cttctacggc aaggtgctgt gcacggatct gccctggctt caggagatcg
      181 gaagacctcg gccgtcgcgg cgcttgccgg tggtgctgac cccggatgaa gtggttcgca
      241 tcctcggttt tctggaaggc gagcatcgtt tgttcgccca gcttctgtat ggaacggctc
      301 gacgtcggaa tggacgatca cactactctt cttttgccta ttataacatg aaaacgcttt
      361 aacagaaaga ataggaaggt atccgactgc tggtattaac cctctttctc aagttatcat
      421 cggcaatgtt gaatggagtc cattcaaaac agcatagctc taaaacatgg aactcaacaa
      481 gtctcagtgt gctgaagttt tgggaccatt caaaacagca tagctctaaa actcgaggtc
      541 attcagactg gctaatgcac ccagtaaggc agcggtatca tcaacggggt ctggtgccac
      601 tttttcaagt tgataacgga ctagccttat tttaacttgc tatttctagc tctaaaacgc
      661 ttggagcgaa cgacctacat ggagaaacag tagagagttg cgataaaaag cgtcaggtag
      721 gatccgctaa tcttatggat aaaaatgcta tggcatagca aagtgtgacg ccgtgcaaat
      781 aatcaatgtg gacttttctg ccgtgattat agacactttt gttacgcgtt tttgtcatgg
      841 ctttggtccc gctttgttac agaatgcttt taataagcgg ggttacc
//
