LOCUS       sequence-147653-        1033 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Donor vector for 3' FLAG tag of human ATF4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1033)
  TITLE     Mendenhall CRISPR tagging plasmids
REFERENCE   2  (bases 1 to 1033)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pFETCh_ATF4
FEATURES             Location/Qualifiers
     source          1..1033
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(869..885)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(878..900)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc
       61 gcatcgcctt ctatcgcctt cttgacgagt tcttctgatt cgaacatcgt caggagcgtc
      121 aatgtgcttg tacatagagt gctgtagctg tgtgttccaa taaattattt tgtagggaaa
      181 gtacttgtgc gtttgaattc ctttcctggc aaagtaaccg agtgaagcgc cacccaccca
      241 ccccaagctg gtcttaacat ttcctgtgag atctggggga ggaggcagtc caaggctgaa
      301 gatcggttcc cagagtggga tttgctcatc cctgctcggt gtaggccacg atggctgatt
      361 catggggccc tggagccgta tagttggcca tttatagtaa gctgggtcct ttgcccagag
      421 ctgagtgctg gttgtgtagg gggaggaggc tggagatctc tgggagattc agagggaggg
      481 acagcctggg cctccacctg tttattcaag agggcttcat gccaacagct gtcgcataaa
      541 accctgaata aggttgcacg gtctttgaga tagcacacaa gattaggttt cacttgttac
      601 ctgaacagtt ctgcagaagc ctcatcctga agaaagaaat ggctcctttt gggaattgat
      661 gctgaaacag gacaggagct gagggttggc ctccgtttct ccttacgggg tggaaggatg
      721 acagcctgcg caggttctgg ctagagctgg gcacaaaggc aggcaacagt tcctgtgaag
      781 gctgttgtgc ctgctgctat ctaaactagg aggcctcttg gaaagtcctc tccactgact
      841 gtagcctcca attcactggc cgtcgttcac tggccgtcgt tttacaacgt cgtgactggg
      901 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
      961 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
     1021 atgcgattta ttc
//
