LOCUS       sequence-147661-         873 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  express hCas9 byT7 promoter with polyadenine tails.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Yoshimi K, Kunihiro Y, Kaneko T, Nagahora H, Voigt B, Mashimo T
  TITLE     ssODN-mediated knock-in with CRISPR-Cas for large genomic regions in
            zygotes.
  JOURNAL   Nat Commun. 2016 Jan 20;7:10431. doi: 10.1038/ncomms10431.
  PUBMED    26786405
REFERENCE   2  (bases 1 to 873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-01-20"; volume: "7:10431. doi"; pages: " 
            10.1038/ncomms10431"
COMMENT     Sequence Label: pCas9-polyA
FEATURES             Location/Qualifiers
     source          1..873
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     13..37
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     111..130
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        111..129
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      137..146
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             149..169
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg
       61 ttttgacctc catagaagac accgggaccg atccagcctc cggactctag taatacgact
      121 cactataggg agagccgcca ccatggcccc aaagaagaag cggaaggtcg gtatccacgg
      181 agtcccagca gccgacaaga agtactccat tgggctcgat atcggcacaa acagcgtcgg
      241 ctgggccgtc attacggacg agtacaaggt gccgagcaaa aaattcaaag ttctgggcaa
      301 taccgatcgc cacagcataa agaagaacct cattggcgcc ctcctgttcg actccgggga
      361 gacggccgaa gccacgcggc tcaaaagaac agcacggcgc agatataccc gcagaaagaa
      421 tcggatctgc tacctgcagg agatctttag taatgagatg gctaaggtgg atgactcttt
      481 cttccatagg ctggaggagt cctttttggt ggaggaggat aaaaagcacg agcgccaccc
      541 aatctttggc aatatcgtgg acgaggtggc gtaccatgaa aagtacccaa ccatatatca
      601 tctgaggaag aagcttgtag acagtactga taaggctgac ttgcggttga tctatctcgc
      661 gctggcgcat atgatcaaat ttcggggaca cttcctcatc gagggggacc tgaacccaga
      721 caacagcgat gtcgacaaac tctttatcca actggttcag acttacaatc agcttttcga
      781 agagaacccg atcaacgcat ccggagttga cgccaaagca atcctgagcg ctaggctgtc
      841 caaatcccgg cggctcgaaa acctcatcgc aca
//
