LOCUS       sequence-147662-        1021 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  express hCas9 byT7 promoter with polyadenine tails.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Yoshimi K, Kunihiro Y, Kaneko T, Nagahora H, Voigt B, Mashimo T
  TITLE     ssODN-mediated knock-in with CRISPR-Cas for large genomic regions in
            zygotes.
  JOURNAL   Nat Commun. 2016 Jan 20;7:10431. doi: 10.1038/ncomms10431.
  PUBMED    26786405
REFERENCE   2  (bases 1 to 1021)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-01-20"; volume: "7:10431. doi"; pages: " 
            10.1038/ncomms10431"
COMMENT     Sequence Label: pCas9-polyA
FEATURES             Location/Qualifiers
     source          1..1021
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(42..62)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gtttaaactc attactaacc ggtagggatc gaaccctttc acaccttcct cttcttcttg
       61 gggtcagccc tgctgtctcc accgagctga gagaggtcga ttcttgtttc atagagcccc
      121 gtaattgact gatgaatcag tgtggcgtcc aggacctcct ttgtagaggt gtaccgcttt
      181 ctgtctatgg tggtgtcgaa gtacttgaag gctgcaggcg cgcccaagtt ggtcagagta
      241 aacaagtgga taatgttttc tgcctgctcc ctgatgggct tatccctgtg cttattgtaa
      301 gcagaaagca ccttatcgag gttagcgtcg gcgaggatca ctcttttgga gaattcgctt
      361 atttgctcga tgatctcatc aaggtagtgt ttgtgttgtt ccacgaacag ctgcttctgc
      421 tcattatctt cgggagaccc tttgagcttt tcatagtggc tggccagata caagaaatta
      481 acgtatttag agggcagtgc cagctcgtta cctttctgca gctcgcccgc actagcgagc
      541 attcgtttcc ggccgttttc aagctcaaag agagagtact tgggaagctt aatgatgagg
      601 tcttttttga cctctttata tcctttcgcc tcgagaaagt cgatggggtt tttttcgaag
      661 cttgatcgct ccatgattgt gatgcccagc agttccttga cgcttttgag ttttttagac
      721 ttccctttct ccactttggc cacaaccagt acactgtaag cgactgtagg agaatcgaat
      781 ccgccgtatt tcttggggtc ccaatctttt ttgcgtgcga tcagcttgtc gctgttcctt
      841 ttcgggagga tactttcctt ggagaagcct ccggtctgta cttcggtctt tttaacgatg
      901 ttcacctgcg gcatggacag gaccttccgg actgtcgcga aatccctacc cttgtcccac
      961 acgatttctc ctgtttctcc gtttgtttcg ataagtggtc gcttccgaat ctctccattg
     1021 g
//
