LOCUS       sequence-147670-        1044 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Murin CD, Segal K, Bryksin A, Matsumura I
  TITLE     Expression vectors for the engineering of genes and genomes in 
            Acinetobacter baylyi ADP1.
  JOURNAL   Appl Environ Microbiol. 2011 Oct 21.
  PUBMED    22020504
REFERENCE   2  (bases 1 to 1044)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2011 Oct 21."
COMMENT     Sequence Label: lacI-PT5-gusA-pBAV1k
FEATURES             Location/Qualifiers
     source          1..1044
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    14..30
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    46..62
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             85..96
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             115..132
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 aaatttattt gctttgtgag cggataacaa ttataataga ttcaattgtg agcggataac
       61 aatttcacac agaattaatt cattaaagag gagaaattaa ccatgggcag cagccatcac
      121 catcatcacc acagccagga tccggccctg agggccttac gtcctgtaga aaccccaacc
      181 cgtgaaatca aaaaactcga cggcctgtgg gcattcagtc tggatcgcga aaactgtgga
      241 attgatcagc gttggtggga aagcgcgtta caagaaagcc gggcaattgc tgtgccaggc
      301 agttttaacg atcagttcgc cgatgcagat attcgtaatt atgcgggcaa cgtctggtat
      361 cagcgcgaag tctttatacc gaaaggttgg gcaggccagc gtatcgtgct gcgtttcgat
      421 gcggtcactc attacggcaa agtgtgggtc aataatcagg aagtgatgga gcatcagggc
      481 ggctatacgc catttgaagc cgatgtcacg ccgtatgtta ttgccgggaa aagtgtacgt
      541 atcaccgttt gtgtgaacaa cgaactgaac tggcagacta tcccgccggg aatggtgatt
      601 accgacgaaa acggcaagaa aaagcagtct tacttccatg atttctttaa ctatgccggg
      661 atccatcgca gcgtaatgct ctacaccacg ccgaacacct gggtggacga tatcaccgtg
      721 gtgacgcatg tcgcgcaaga ctgtaaccac gcgtctgttg actggcaggt ggtggccaat
      781 ggtgatgtca gcgttgaact gcgtgatgcg gatcaacagg tggttgcaac tggacaaggc
      841 actagcggga ctttgcaagt ggtgaatccg cacctctggc aaccgggtga aggttatctc
      901 tatgaactgt gcgtcacagc caaaagccag acagagtgtg atatctaccc gcttcgcgtc
      961 ggcatccggt cagtggcagt gaagggcgaa cagttcctga ttaaccacaa accgttctac
     1021 tttactggct ttggtcgtca tgaa
//
