LOCUS       sequence-147671-        1100 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Murin CD, Segal K, Bryksin A, Matsumura I
  TITLE     Expression vectors for the engineering of genes and genomes in 
            Acinetobacter baylyi ADP1.
  JOURNAL   Appl Environ Microbiol. 2011 Oct 21.
  PUBMED    22020504
REFERENCE   2  (bases 1 to 1100)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2011 Oct 21."
COMMENT     Sequence Label: lacI-PT5-gusA-pBAV1k
FEATURES             Location/Qualifiers
     source          1..1100
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(592..597,598..615,616..622)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    630..646
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     654..670
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     654..670
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(686..703)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(686..702)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(695..717)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     860..878
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(902..916,917..922,923..939,940..946)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    922..938
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    954..970
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             993..1004
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             1023..1040
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 caaatcgcgc tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg tctggctggc
       61 tggcataaat atctcactcg caatcaaatt cagccgatag cggaacggga aggcgactgg
      121 agtgccatgt ccggttttca acaaaccatg caaatgctga atgagggcat cgttcccact
      181 gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc
      241 gggctgcgcg ttggtgcgga tatctcggta gtgggatacg acgataccga agacagctca
      301 tgttatatcc cgccgttaac caccatcaaa caggattttc gcctgctggg gcaaaccagc
      361 gtggaccgct tgctgcaact ctctcagggc caggcggtga agggcaatca gctgttgccc
      421 gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc
      481 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
      541 tgagcgcaac gcaattaatg taagttagct cactcattag gcaccccagg ctttacactt
      601 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
      661 cagctatgac catgattacg gattcactgg ccgtcgtttt acaacgtcgt gactgggaaa
      721 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
      781 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcctaagaaa ccattattat
      841 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtcttc acctcgagaa
      901 atcataaaaa atttatttgc tttgtgagcg gataacaatt ataatagatt caattgtgag
      961 cggataacaa tttcacacag aattaattca ttaaagagga gaaattaacc atgggcagca
     1021 gccatcacca tcatcaccac agccaggatc cggccctgag ggccttacgt cctgtagaaa
     1081 ccccaacccg tgaaatcaaa
//
