LOCUS       sequence-147672-        1009 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Murin CD, Segal K, Bryksin A, Matsumura I
  TITLE     Expression vectors for the engineering of genes and genomes in 
            Acinetobacter baylyi ADP1.
  JOURNAL   Appl Environ Microbiol. 2011 Oct 21.
  PUBMED    22020504
REFERENCE   2  (bases 1 to 1009)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2011 Oct 21."
COMMENT     Sequence Label: lacI-PT5-gusA-pBAV1k
FEATURES             Location/Qualifiers
     source          1..1009
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    327..346
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     promoter        373..450
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     primer_bind     complement(470..489)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 ggatttcatt ttctcccacc agcttatata ccttagcagg agacattcct tccgtatctt
       61 ttacgcagcg gtattcttcg atcagttttt tcaattccgg tgatattctc attttagcca
      121 tttattattt ccttcctctt ttctacagta tttaaagata ccccaagaag ctaattataa
      181 caagacgaac tccaattcac tgttccttgc attctaaaac cttaaataca gaaaacagcc
      241 ttttcaaagt tgttttcaaa gttggcgtat aacatagtat cgacggagcc gattttgaaa
      301 ccacaattat gatagaattt gacgtcgaat tcgcggccgc ttctagaact atagctagca
      361 tgcatttacg ttgacaccat cgaatggtgc aaaacctttc gcggtatggc atgatagcgc
      421 ccggaagaga gtcaattcag ggtggtgaat gtgaaaccag taacgttata cgatgtcgca
      481 gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt
      541 tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta cattcccaac
      601 cgcgtggcac aacaactggc gggcaaacag tcgttgctga ttggcgttgc cacctccagt
      661 ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg
      721 ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg
      781 gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca ttaactatcc gctggatgac
      841 caggatgcca ttgctgtgga agctgcctgc actaatgttc cggcgttatt tcttgatgtc
      901 tctgaccaga cacccatcaa cagtattatt ttctcccatg aagacggtac gcgactgggc
      961 gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc tgttagcgg
//
