LOCUS       sequence-147675-        1001 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Murin CD, Segal K, Bryksin A, Matsumura I
  TITLE     Expression vectors for the engineering of genes and genomes in 
            Acinetobacter baylyi ADP1.
  JOURNAL   Appl Environ Microbiol. 2011 Oct 21.
  PUBMED    22020504
REFERENCE   2  (bases 1 to 1001)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2011 Oct 21."
COMMENT     Sequence Label: araC-pBAD-gusA-pBAV1k
FEATURES             Location/Qualifiers
     source          1..1001
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(284..301)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        complement(347..631)
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     complement(405..424)
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
ORIGIN
        1 aacgcagcac gatacgctgg cctgcccaac ctttcggtat aaagacttcg cgctgatacc
       61 agacgttgcc cgcataatta cgaatatctg catcggcgaa ctgatcgtta aaactgcctg
      121 gcacagcaat tgcccggctt tcttgtaacg cgctttccca ccaacgctga tcaattccac
      181 agttttcgcg atccagactg aatgcccaca ggccgtcgag ttttttgatt tcacgggttg
      241 gggtttctac aggacgtaag gccctcaggg ccggatcctg gctgtggtga tgatggtgat
      301 ggctgctgcc catggttaat tcctcctgtt agcccaaaaa acgggtatgg agaaacagta
      361 gagagttgcg ataaaaagcg tcaggtagga tccgctaatc ttatggataa aaatgctatg
      421 gcatagcaaa gtgtgacgcc gtgcaaataa tcaatgtgga cttttctgcc gtgattatag
      481 acacttttgt tacgcgtttt tgtcatggct ttggtcccgc tttgttacag aatgctttta
      541 ataagcgggg ttaccggttt ggttagcgag aagagccagt aaaagacgca gtgacggcaa
      601 tgtctgatgc aatatggaca attggtttct tctctgaatg gcgggagtat gaaaagtatg
      661 gctgaagcgc aaaatgatcc cctgctgccg ggatactcgt ttaatgccca tctggtggcg
      721 ggtttaacgc cgattgaggc caacggttat ctcgattttt ttatcgaccg accgctggga
      781 atgaaaggtt atattctcaa tctcaccatt cgcggtcagg gggtggtgaa aaatcaggga
      841 cgagaatttg tttgccgacc gggtgatatt ttgctgttcc cgccaggaga gattcatcac
      901 tacggtcgtc atccggaggc tcgcgaatgg tatcaccagt gggtttactt tcgtccgcgc
      961 gcctactggc atgaatggct taactggccg tcaatatttg c
//
