LOCUS       sequence-147676-         984 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 984)
  AUTHORS   Murin CD, Segal K, Bryksin A, Matsumura I
  TITLE     Expression vectors for the engineering of genes and genomes in 
            Acinetobacter baylyi ADP1.
  JOURNAL   Appl Environ Microbiol. 2011 Oct 21.
  PUBMED    22020504
REFERENCE   2  (bases 1 to 984)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2011 Oct 21."
COMMENT     Sequence Label: lacI-PT7-gusA-pBAV1k
FEATURES             Location/Qualifiers
     source          1..984
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(281..298)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    354..378
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(378..397)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(379..397)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        443..520
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     complement(540..559)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 gcagcacgat acgctggcct gcccaacctt tcggtataaa gacttcgcgc tgataccaga
       61 cgttgcccgc ataattacga atatctgcat cggcgaactg atcgttaaaa ctgcctggca
      121 cagcaattgc ccggctttct tgtaacgcgc tttcccacca acgctgatca attccacagt
      181 tttcgcgatc cagactgaat gcccacaggc cgtcgagttt tttgatttca cgggttgggg
      241 tttctacagg acgtaaggcc ctcagggccg gatcctggct gtggtgatga tggtgatggc
      301 tgctgcccat ggtatatctc cttattaaag ttaaacaaaa ttatttctac aggggaattg
      361 ttatccgctc acaattcccc tatagtgagt cgtattaatt tcctaatgca ggagtcgcat
      421 aagggagagc gtcgagatcc cggacaccat cgaatggcgc aaaacctttc gcggtatggc
      481 atgatagcgc ccggaagaga gtcaattcag ggtggtgaat gtgaaaccag taacgttata
      541 cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc
      601 cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta
      661 cattcccaac cgcgtggcac aacaactggc gggcaaacag tcgttgctga ttggcgttgc
      721 cacctccagt ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc
      781 cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg
      841 taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca ttaactatcc
      901 gctggatgac caggatgcca ttgctgtgga agctgcctgc actaatgttc cggcgttatt
      961 tcttgatgtc tctgaccaga cacc
//
