LOCUS       sequence-147693-         795 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  LentiCRISPR-mCherry with sgRNA targeting human MLKL.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 795)
  AUTHORS   McComb S, Aguade-Gorgorio J, Harder L, Marovca B, Cario G, Eckert C,
            Schrappe M, Stanulla M, von Stackelberg A, Bourquin JP, Bornhauser 
            BC
  TITLE     Activation of concurrent apoptosis and necroptosis by SMAC mimetics 
            for the treatment of refractory and relapsed ALL.
  JOURNAL   Sci Transl Med. 2016 May 18;8(339):339ra70. doi: 
            10.1126/scitranslmed.aad2986.
  PUBMED    27194728
REFERENCE   2  (bases 1 to 795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Transl 
            Med."; date: "2016-05-18"; volume: "8(339):339ra70. doi"; pages: " 
            10.1126/scitranslmed.aad2986"
COMMENT     Sequence Label: pLC-mCherry-MLKL
FEATURES             Location/Qualifiers
     source          1..795
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(25..273)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(83..102)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(253..273)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     CDS             complement(451..495)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 aacttagcag aatccacggg gatcggtgtt tcgtcctttc cacaagatat ataaagccaa
       61 gaaatcgaaa tactttcaag ttacggtaag catatgatag tccattttaa aacataattt
      121 taaaactgca aactacccaa gaaattatta ctttctacgt cacgtatttt gtactaatat
      181 ctttgtgttt acagtcaaat taattctaat tatctctcta acagccttgt atcgtatatg
      241 caaatatgaa ggaatcatgg gaaataggcc ctctctgggt cccctcgggg ttgggaggtg
      301 ggtctgaaac gataatggtg aatatccctg cctaactcta ttcactatag aaagtacagc
      361 aaaaactatt cttaaaccta ccaagcctcc tactatcatt atgaataatt ttatatacca
      421 cagccaattt gttatgttaa accaattcca caaacttgcc catttatcta attccaataa
      481 ttcttgttca ttcttttctt gctggttttg cgattcttca attaaggagt gtattaagct
      541 tgtgtaattg ttaatttctc tgtcccactc catccaggtc gtgtgattcc aaatctgttc
      601 cagagattta ttactccaac tagcattcca aggcacagca gtggtgcaaa tgagttttcc
      661 agagcaaccc caaatcccca ggagctgttg atcctttagg tatctttcca cagccaggat
      721 tcttgcctgg agctgcttga tgccccagac tgtgagttgc aacagatgct gttgcgcctc
      781 aatagccctc agcaa
//
