LOCUS       sequence-147702-         825 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the ketosynthase FabF/KASII from the E. coli fatty acid 
            synthase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Worthington AS, Rivera H, Torpey JW, Alexander MD, Burkart MD
  TITLE     Mechanism-based protein cross-linking probes to investigate carrier 
            protein-mediated biosynthesis.
  JOURNAL   ACS Chem Biol. 2006 Dec 20;1(11):687-91.
  PUBMED    17184132
REFERENCE   2  (bases 1 to 825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Chem 
            Biol."; date: "2006-12-20"; volume: "1(11)"; pages: "687-91"
COMMENT     Sequence Label: FabF (KASII) pet28 N-terminal His Tag
FEATURES             Location/Qualifiers
     source          1..825
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(15..32)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gcagccggat ctcagtggtg gtggtggtgg tgctcgagtg cggccgcaag cttgtcgacg
       61 gagctcgaat tcggatcctt agatcttttt aaagatcaaa gaaccattag tgccaccgaa
      121 gccgaaggag ttacacagag tgtattccat tccgctaacc tgacgcgctt cgtgcggtac
      181 gaaatccaga tcgcaacctt catccgggtt atccaggttg atggttggcg gaacagcctg
      241 atcgcgcagc gccaggatgg agtagataga ttctactgca cccgccgcac ctaacaggtg
      301 accggtcata gatttcgtgg agcttaccaa cacacggctt gcagcttcac cgaagatggt
      361 tttcaccgcc tgcgcttcag ctttatcgcc agccggcgta gaagtaccgt gcgcgttaac
      421 gtagccaatc tgactcgctt caatgcctgc atcacgcaga gcatttgcca tcgccagagc
      481 tgcgcctgcg ccattttctg gcggtgacgt catatgataa gcatcgctgc tcataccaaa
      541 gccgacgagt tcagcgtaaa ttttcgcacc gcgttttttc gcgtgttcgt actcttcaag
      601 taccagcata ccggcaccat cgcccagtac gaaaccatca cgctctttat cccacgggcg
      661 gctcgccgct tgcgggttat cattgcgggt agataatgca cgtgccgcgc caaaaccacc
      721 aacgcccagc ggcgtactgg ctttctctgc gccacctgca accatcacgt cagcatcgcc
      781 atacgcgata atacgcgcag catggccaat gttgtgcacg ccgga
//
