LOCUS       sequence-147754-        1857 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FRET biosensor. Visualization of FAK activity at lipid rafts in live
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Seong J, Ouyang M, Kim T, Sun J, Wen PC, Lu S, Zhuo Y, Llewellyn NM,
            Schlaepfer DD, Guan JL, Chien S, Wang Y
  TITLE     Detection of focal adhesion kinase activation at membrane 
            microdomains by fluorescence resonance energy transfer.
  JOURNAL   Nat Commun. 2011 Jul 26;2:406. doi: 10.1038/ncomms1414.
  PUBMED    21792185
REFERENCE   2  (bases 1 to 1857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2011-07-26"; volume: "2:406. doi"; pages: " 
            10.1038/ncomms1414"
COMMENT     Sequence Label: Lyn FAK biosensor
FEATURES             Location/Qualifiers
     source          1..1857
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(64..66,67..69,70..582)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIE"
     primer_bind     complement(109..130)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(370..389)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     717..738
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1135..1845
                     /codon_start=1
                     /product="yellow fluorescent protein optimized for FRET 
                     with CyPet (Nguyen and Daugherty, 2005)"
                     /label=YPet
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKL
                     LCTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKAN
                     FKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALFKDPNEKRDHMVLLEF
                     LTAAGITEGMNELYK"
ORIGIN
        1 atgggctgca tcaagagcaa gcgcaaggac aacctgaacg acgacggcgt ggacatgaag
       61 accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
      121 gacggcgacg taaacggcca caggttcagc gtgtccggcg agggcgaggg cgatgccacc
      181 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
      241 accctcgtga ccaccctgac ctggggcgtg cagtgcttca gccgctaccc cgaccacatg
      301 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgtaccatc
      361 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
      421 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
      481 cacaagctgg agtacaacta catcagccac aacgtctata tcaccgccga caagcagaag
      541 aacggcatca aggcccactt caagatccgc cacaacatcg aggacggcag cgtgcagctc
      601 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
      661 cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg
      721 gtcctgctgg agttcgtgac cgccgcccgc atgcattggt attttgggaa gatcactcgt
      781 cgggagtccg agcggctgct gctcaacccc gaaaaccccc ggggaacctt cttggtccgg
      841 gagagcgaga cgacaaaagg tgcctattgc ctctccgttt ctgactttga caacgccaag
      901 gggctcaatg tgaagcacta caagatccgc aagctggaca gcggcggctt ctacatcacc
      961 tcacgcacac agttcagcag cctgcagcag ctggtggcct actactccaa acatgctgat
     1021 ggcttgtgcc accgcctgac taacgtctgt gggtctacat ctggatctgg gaagccgggt
     1081 tctggtgagg gttctgagac cgacgactac gctgagatca tcgacgagga gctctctaaa
     1141 ggtgaagaat tattcactgg tgttgtccca attttggttg aattagatgg tgatgttaat
     1201 ggtcacaaat tttctgtctc cggtgaaggt gaaggtgatg ctacgtacgg taaattgacc
     1261 ttaaaattac tctgtactac tggtaaattg ccagttccat ggccaacctt agtcactact
     1321 ttaggttatg gtgttcaatg ttttgctaga tacccagatc atatgaaaca acatgacttt
     1381 ttcaagtctg ccatgccaga aggttatgtt caagaaagaa ctattttttt caaagatgac
     1441 ggtaactaca agaccagagc tgaagtcaag tttgaaggtg ataccttagt taatagaatc
     1501 gaattaaaag gtattgattt taaagaagat ggtaacattt taggtcacaa attggaatac
     1561 aactataact ctcacaatgt ttacatcact gctgacaaac aaaagaatgg tatcaaagct
     1621 aacttcaaaa ttagacacaa cattgaagat ggtggtgttc aattagctga ccattatcaa
     1681 caaaatactc caattggtga tggtccagtc ttgttaccag acaaccatta cttatcctat
     1741 caatctgcct tattcaaaga tccaaacgaa aagagagacc acatggtctt gttagaattt
     1801 ttgactgctg ctggtattac cgagggtatg aatgaattgt acaaatagta agaattc
//
