LOCUS       sequence-147755-         944 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral empty cloning vector containing IRES and GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 944)
  AUTHORS   Caviness K, Cicchini L, Rak M, Umashankar M, Goodrum F
  TITLE     Complex expression of the UL136 gene of human cytomegalovirus 
            results in multiple protein isoforms with unique roles in 
            replication.
  JOURNAL   J Virol. 2014 Dec;88(24):14412-25. doi: 10.1128/JVI.02711-14. Epub 
            2014 Oct 8.
  PUBMED    25297993
REFERENCE   2  (bases 1 to 944)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/JVI.02711-14"; journalName: "J Virol"; date: "2014-12"; 
            volume: "88"; issue: "24"; pages: "14412-25"
COMMENT     Sequence Label: pCIG3 (pCMV-IRES-GFP version 3)
FEATURES             Location/Qualifiers
     source          1..944
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     2..26
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    join(177..750,751..753,754..762)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(343..360)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     570..589
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     regulatory      770..779
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(821..842)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc
       61 atagaagatt ctagagctag cgaattcggt gatcagggta tacggctcga ggggatatcg
      121 ggtttaaacg gggcgcgccg ggcgatcgcg gttcgaaggt taattaaggg gatccgcccc
      181 tctccctccc cccccctaac gttactggcc gaagccgctt ggaataaggc cggtgtgcgt
      241 ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac
      301 ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc
      361 aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa
      421 cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg
      481 gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg
      541 tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc
      601 tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat
      661 gctttacatg tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac cacggggacg
      721 tggttttcct ttgaaaaaca cgatgataag cttgccacaa ccagatctcg ccaccatggt
      781 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
      841 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
      901 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgc
//
