LOCUS       sequence-147763-         933 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Rab5 Constitutively active (Q79L) expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 933)
  TITLE     Goodrum lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCIG3 Rab5CA
FEATURES             Location/Qualifiers
     source          1..933
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      18..27
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    join(35..43,44..46,47..621)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(208..227)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     437..454
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 ctcctcgccc ttgctcacca tggtggcgag atctggttgt ggcaagctta tcatcgtgtt
       61 tttcaaagga aaaccacgtc cccgtggttc ggggggccta gacgtttttt taacctcgac
      121 taaacacatg taaagcatgt gcaccgaggc cccagatcag atcccataca atggggtacc
      181 ttctgggcat ccttcagccc cttgttgaat acgcttgagg agagccattt gactctttcc
      241 acaactatcc aactcacaac gtggcactgg ggttgtgccg cctttgcagg tgtatcttat
      301 acacgtggct tttggccgca gaggcacctg tcgccaggtg gggggttccg ctgcctgcaa
      361 agggtcgcta cagacgttgt ttgtcttcaa gaagcttcca gaggaactgc ttccttcacg
      421 acattcaaca gaccttgcat tcctttggcg agaggggaaa gacccctagg aatgctcgtc
      481 aagaagacag ggccaggttt ccgggccctc acattgccaa aagacggcaa tatggtggaa
      541 aataacatat agacaaacgc acaccggcct tattccaagc ggcttcggcc agtaacgtta
      601 gggggggggg agggagaggg gcggatcctc agttactaca acactgattc ctggttggtt
      661 gtgtgggttc ggtaaggtct actcctcttc ctctggcaga atttgctcct ggattttgtg
      721 gttcattctt tggcaatttt ttagctattg ccatgaatat ttcatttaca ttcattgatg
      781 ttttagcgga tgtctccatg aataataaac tattgtcatc tgcataggac tgtgcttcct
      841 ggaaatctac tgctctttta tttgctaggt cggccttgtt tcccgataaa gctattacaa
      901 tgttaggact tgcttgcctc tgaagttctt taa
//
