LOCUS       sequence-147765-        6209 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of exogenous SL1 in C. elegans muscle.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6209)
  AUTHORS   Ma X, Zhan G, Sleumer MC, Chen S, Liu W, Zhang MQ, Liu X
  TITLE     Analysis of C. elegans muscle transcriptome using 
            trans-splicing-based RNA tagging (SRT).
  JOURNAL   Nucleic Acids Res. 2016 Aug 23. pii: gkw734.
  PUBMED    27557708
REFERENCE   2  (bases 1 to 6209)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Aug 23. pii: gkw734."
COMMENT     Sequence Label: Pmyo3::tag:sl1(G16C)
FEATURES             Location/Qualifiers
     source          1..6209
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(11..28)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(182..770)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(262..281)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(941..1732,1733..1801))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1564..1583
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     CDS             complement(1819..2613)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1930..1949)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     2540..2559
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     rep_origin      complement(2957..3385)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3067..3088)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3279..3298
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3511..3533
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3525..3542
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3526..3542
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3549..3568
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3549..3567
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5868..5884)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5869..5885)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(5910..5930)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5910..5928)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5949..5965)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5989..6005)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6002..6024)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6013..6029
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6037..6043,6044..6061,6062..6067))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6082..6103
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
       61 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
      121 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
      181 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
      241 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
      301 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
      361 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
      421 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
      481 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
      541 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
      601 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
      661 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
      721 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
      781 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
      841 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
      901 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
      961 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     1021 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     1081 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     1141 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     1201 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     1261 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     1321 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     1381 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     1441 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     1501 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     1561 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     1621 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     1681 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     1741 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     1801 tactcttcct ttttcaattc agaagaactc gtcaagaagg cgatagaagg cgatgcgctg
     1861 cgaatcggga gcggcgatac cgtaaagcac gaggaagcgg tcagcccatt cgccgccaag
     1921 ctcttcagca atatcacggg tagccaacgc tatgtcctga tagcggtccg ccacacccag
     1981 ccggccacag tcgatgaatc cagaaaagcg gccattttcc accatgatat tcggcaagca
     2041 ggcatcgccg tgggtcacga cgagatcctc gccgtcgggc atgcgcgcct tgagcctggc
     2101 gaacagttcg gctggcgcga gcccctgatg ctcttcgtcc agatcatcct gatcgacaag
     2161 accggcttcc atccgagtac gtgctcgctc gatgcgatgt ttcgcttggt ggtcgaatgg
     2221 gcaggtagcc ggatcaagcg tatgcagccg ccgcattgca tcagccatga tggatacttt
     2281 ctcggcagga gcaaggtggg atgacaggag atcctgcccc ggcacttcgc ccaatagcag
     2341 ccagtccctt cccgcttcag tgacaacgtc gagcacagct gcgcaaggaa cgcccgtcgt
     2401 ggccagccac gatagccgcg ctgcctcgtc ctgcagttca ttcagggcac cggacaggtc
     2461 ggtcttgaca aaaagaaccg ggcgcccctg cgctgacagc cggaacacgg cggcatcaga
     2521 gcagccgatt gtctgttgtg cccagtcata gccgaatagc ctctccaccc aagcggccgg
     2581 agaacctgcg tgcaatccat cttgttcaat catgcgaaac gatcctcatc ctgtctcttg
     2641 atcagatctt gatcccctgc gccatcagat ccttggcggc aagaaagcca tccagtttac
     2701 tttgcagggc ttcccaacct taccagaggg cgccccagct ggcaattccg gttcgcttgc
     2761 tgtccataaa accgcccagt ctagctatcg ccatgtaagc ccactgcaag ctacctgctt
     2821 tctctttgcg cttgcgtttt cccttgtcca gatagcccag tagctgacat tcatccgggg
     2881 tcagcaccgt ttctgcggac tggctttcta cgtgttccgc ttcctttagc agcccttgcg
     2941 ccctgaattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa
     3001 taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt
     3061 gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg
     3121 cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt
     3181 ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga
     3241 gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg
     3301 ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg
     3361 cttaatgcgc cgctacaggg cgcgtccatt cgccattcag gctgcgcaac tgttgggaag
     3421 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
     3481 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
     3541 gtgaattgta atacgactca ctatagggcg aattgaagct gcccttgctc gagcggctat
     3601 aataagttct tgaataaaat aattttcccg acaaaacatg agtatttctt tcgaaaataa
     3661 aagtgcaggc taattagaga ttattctgta attaactgca taatttgtca cgtgccatag
     3721 ttttacattc cactacgtca tagttcttaa aatactaatc tcctgaaaat agaagtaggt
     3781 gaagaaagtt taattatcag ttctaaaatg acaattgatc tttggaatat gttctgaaac
     3841 taccgatcat tgaacagatg ctatttgaat gatatagaat tgtatatttg caatttctga
     3901 aacgcgttct taaaggcaca cagattaatt caaaagggtc tggccgcaaa aaggtttatg
     3961 gtggccgatt ttgagttttg tgtgtgattg ctttttcaca atcagtgttt tcaggattat
     4021 gtgatgaact agatcttcaa gtttcgttac atttcatatg ttttcggaac tcacgaagta
     4081 catattgggt attgtgctca aaaaattcag caatcagctt cgctccgctg actttagaac
     4141 ccaaaaaaat agtatggcca aactgactgt gttacgatca tttcaatttt tcaatacata
     4201 tttaagattt ctaagagtaa gaaggtcaaa aactgttctg gaatacatat atatttttca
     4261 ggttacaatt agtcaaaaag tgcactgaaa tatacgtttt aatttcacga ataacccaat
     4321 tagttcaatg tatttttggt caaccaacgt taaagtttgg cttccaacca attatcattt
     4381 ctgatcaacc acaatgtttt ttctttatct gcaagttaat tttttatttt tatccagatg
     4441 tttggcatat ttttcaattc ttcactagcg cccacttctt gcacttccgg cgccctgaat
     4501 ctaatgcatc tgttgcaaga attgaaagac caatcaacac attgttttct tcacgagata
     4561 ctgaagaaaa tgaataaaaa cagagaaaaa gagccatgtg attagtgaca actgttgcta
     4621 acagatacca tagcttggac ttggtacgtg atggcaacgt atgggtcaac aaaaatgatt
     4681 gcagaggggg tgcaaaacag tcaagtcgag aaaatatgaa aaacagaaaa caaagaacag
     4741 aaaaatgggt ttgagagtca gtataattta taaaagaaaa attgtacata gaaattaacc
     4801 atttttgtag aagaagttat ttttcaagca tcgttaaaaa ttattcaaag caccttattt
     4861 catatttaat tttaaacatg gttaaatgaa caacacggtg cgcaatcagg aaaacttgaa
     4921 atctgaaact gttgttgtga tcttcttcgc aactgttcag atagcactag tgtaatgtta
     4981 agagtgcgcg aatataatgg aatataatgg atcacacctc ctgccatcag gtaaacgtct
     5041 ctgttatcac atatttccaa ctattaaatt tttacctttt acagttttac atttttttga
     5101 aaaaagtaac tttttgtctt caaaatccct gacgaaaata tcaaatattt taatcgagac
     5161 tgcagaggaa ccgattgatg atttggaaaa tccagcttta cctgtgtaag aactgaaaag
     5221 tttcataacc ctagggtatt cccagttaca ttccccactg gctaacaata gcacccagtt
     5281 tttcatcacc tttcttcaaa tttctcggcg atttgttaaa aacaaaattt gtgtcccttc
     5341 tctgatatct ctatgtctct aaacacaagt tcatcggaaa acgaaggagg gtaggtgttg
     5401 gttgggctcc cgaagtgaaa atagaagagc aagaatagaa tattagagag agagtgcaga
     5461 gagggcggga tagctcccgg gattccgttt tcttcttctt tatcttcaac gatgatgtgt
     5521 gtgcgtgttg tatagattct gttgctcccc cacaactcgc tccgaaggct caatacaatt
     5581 caattacacg acgctcttcc gatctggttt aattacccaa ctttgaggta aacattgaaa
     5641 ctgacccaaa gaaatttggc gttagctata aattttggaa cgtctcctct cggggagaca
     5701 aaaatactaa tagggataac agggtaatga aattcgaaaa tcggttcatc catgttggag
     5761 aacggtccat tcgaaaatat gtggagatga gggagcatgg ctgtccgtat gtggagatgt
     5821 gggaaggtga tgatattatt ggggaacgaa ttcgatatca agcttatcga taccgtcgac
     5881 ctcgagggcc cggtacccag cttttgttcc ctttagtgag ggttaattgc gcgcttggcg
     5941 taatcatggt catagctgtt tcctgaaggg cagcttggcg taatcatggt catagctgtt
     6001 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     6061 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     6121 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     6181 ggggagaggc ggtttgcgta ttgggcgct
//
