LOCUS       sequence-147766-         771 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre-dependent Cas9 expression plasmid and targeting vector for the 
            mouse Rosa26 locus. Cas9 expression is Cre-dependent and the 
            targeting vector has positive (Neo) and negative (DTA) selection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Platt RJ, Chen S, Zhou Y, Yim MJ, Swiech L, Kempton HR, Dahlman JE, 
            Parnas O, Eisenhaure TM, Jovanovic M, Graham DB, Jhunjhunwala S, 
            Heidenreich M, Xavier RJ, Langer R, Anderson DG, Hacohen N, Regev A,
            Feng G, Sharp PA, Zhang F
  TITLE     CRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling.
  JOURNAL   Cell. 2014 Sep 24. pii: S0092-8674(14)01163-5. doi: 
            10.1016/j.cell.2014.09.014.
  PUBMED    25263330
REFERENCE   2  (bases 1 to 771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2014 
            Sep 24. pii: S0092-8674(14)01163-5. doi: 
            10.1016/j.cell.2014.09.014."
COMMENT     Sequence Label: LSL-Cas9-Rosa26TV
FEATURES             Location/Qualifiers
     source          1..771
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     225..246
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(289..345)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(355..402)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 gcgctcctgg acgtagcctt cgggcatggc ggacttgaag aagtcgtgct gcttcatgtg
       61 gtcggggtag cggctgaagc actgcacgcc gtaggtcagg gtggtcacga gggtgggcca
      121 gggcacgggc agcttgccgg tggtgcagat gaacttcagg gtcagcttgc cgtaggtggc
      181 atcgccctcg ccctcgccgg acacgctgaa cttgtggccg tttacgtcgc cgtccagctc
      241 gaccaggatg ggcaccaccc cggtgaacag ctcctcgccc ttgctcactg gcccgggatt
      301 ctcttcgaca tcccctgctt gtttcaacag ggagaagtta gtggctccgc ttcccttttt
      361 cttttttgcc tggccggcct ttttcgtggc cgccggcctt ttgtcgcctc ccagctgaga
      421 caggtcgatc cgtgtctcgt acaggccggt gatgctctgg tggatcaggg tggcgtccag
      481 cacctctttg gtgctggtgt acctcttccg gtcgatggtg gtgtcaaagt acttgaaggc
      541 ggcaggggct cccagattgg tcagggtaaa caggtggatg atattctcgg cctgctctct
      601 gatgggctta tcccggtgct tgttgtaggc ggacagcact ttgtccagat tagcgtcggc
      661 caggatcact ctcttggaga actcgctgat ctgctcgatg atctcgtcca ggtagtgctt
      721 gtgctgttcc acaaacagct gtttctgctc attatcctcg ggggagccct t
//
