LOCUS       sequence-147798-         752 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral expression plasmid encoding wild type human BRD9 with a C
            terminal 3x FLAG-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Hohmann AF, Martin LJ, Minder JL, Roe JS, Shi J, Steurer S, Bader G,
            McConnell D, Pearson M, Gerstberger T, Gottschamel T, Thompson D, 
            Suzuki Y, Koegl M, Vakoc CR
  TITLE     Sensitivity and engineered resistance of myeloid leukemia cells to 
            BRD9 inhibition.
  JOURNAL   Nat Chem Biol. 2016 Jul 4. doi: 10.1038/nchembio.2115.
  PUBMED    27376689
REFERENCE   2  (bases 1 to 752)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2016 Jul 4. doi: 10.1038/nchembio.2115."
COMMENT     Sequence Label: MSCV-Cterm_3xFL_BRD9-PGK-Puro-IRES-GFP
FEATURES             Location/Qualifiers
     source          1..752
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(50..115)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             complement(606..617)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 ctccagactg ccttgggaaa agcgcctccc ctacccggta gaattctcac ttgtcatcgt
       61 catccttgta atcgatatca tgatctttat aatcaccgtc atggtctttg taatcgcctc
      121 cggtcttggc agaggccgca ggctctggag actgaagaaa ctcatagggg tcgtgggtga
      181 cgtctggctg ctccccgaca ctcaggcgag aagggcttcc caggtggtgc tggtccctct
      241 cggaggcgtt ggacagggag ctgaggttgg acgacggccg agagccgccg cgctccgcct
      301 gtgcttcgtg caggtcctgc aggagcttcg tcgtctcatc caagttcaaa tggctgtcgt
      361 cagggtccag ctccttcttc accttcccca gagagctgag catggagata tccacagaaa
      421 cgtcgggata ggacttcatc gacatgaact ccagaacaga gctgctgctg tctcctaggg
      481 tgtccccaac cttggcttca tctggaggct tcatgggaac atttcttctc tgcttcagct
      541 ggaagagcgt cctagagtgg tctccgcctg tgatctggtc caggaggtcg tccaccactt
      601 tcttgctgta gctcccagca tccttcacaa actcctgcag gctcagcgca cactgcacgc
      661 ctgtctcatc tccgtaggct gagtagagca gctccatctc gtccgacttc aagtcgccaa
      721 atactgaatt attctgcatc gaaagcgcag ta
//
