LOCUS       sequence-147803-         771 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes the expression of Luciferase and mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Mallampati S, Sun B, Lu Y, Ma H, Gong Y, Wang D, Lee JS, Lin K, Sun 
            X
  TITLE     Integrated genetic approaches identify the molecular mechanisms of 
            Sox4 in early B-cell development: intricate roles for RAG1/2 and 
            CK1epsilon.
  JOURNAL   Blood. 2014 Jun 26;123(26):4064-76. doi: 
            10.1182/blood-2013-12-543801. Epub 2014 Apr 30.
  PUBMED    24786772
REFERENCE   2  (bases 1 to 771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1182/blood-2013-12-543801"; journalName: "Blood"; date: 
            "2014-06-26- 26"; volume: "123"; issue: "26"; pages: "4064-76"
COMMENT     Sequence Label: MI-Luciferase-IRES-mCherry
FEATURES             Location/Qualifiers
     source          1..771
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      142..151
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(293..311)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     561..580
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ctgatctggg gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaaacgtc
       61 taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgatga taatatggcc
      121 acaaccatgg atccaccggt cgccaccatg gtgagcaagg gcgaggagga taacatggcc
      181 atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag
      241 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg
      301 aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg
      361 tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc
      421 ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc
      481 gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc
      541 accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc
      601 tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag
      661 ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc
      721 gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc c
//
