LOCUS       sequence-147805-         910 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral expression plasmid encoding shBrd9_1061 (targeting murine
            Brd9).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Hohmann AF, Martin LJ, Minder JL, Roe JS, Shi J, Steurer S, Bader G,
            McConnell D, Pearson M, Gerstberger T, Gottschamel T, Thompson D, 
            Suzuki Y, Koegl M, Vakoc CR
  TITLE     Sensitivity and engineered resistance of myeloid leukemia cells to 
            BRD9 inhibition.
  JOURNAL   Nat Chem Biol. 2016 Jul 4. doi: 10.1038/nchembio.2115.
  PUBMED    27376689
REFERENCE   2  (bases 1 to 910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2016 Jul 4. doi: 10.1038/nchembio.2115."
COMMENT     Sequence Label: MSCV-miR30_shBrd9_1061-PGK-NeoR-IRES-GFP
FEATURES             Location/Qualifiers
     source          1..910
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           66..187
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           215..229
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           257..383
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     primer_bind     complement(448..471)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     845..864
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
ORIGIN
        1 cagccctcac tccttctcta ggcgccggaa ttagatctct cgactaggga taacagggta
       61 attgtttgaa tgaggcttca gtactttaca gaatcgttgc ctgcacatct tggaaacact
      121 tgctgggatt acttcttcag gttaacccaa cagaaggctc gagaaggtat attgctgttg
      181 acagtgagcg cggacctgag ttcactgtct aatagtgaag ccacagatgt attagacagt
      241 gaactcaggt ccatgcctac tgcctcggaa ttcaaggggc tactttagga gcaattatct
      301 tgtttactaa aactgaatac cttgctatct ctttgataca tttttacaaa gctgaattaa
      361 aatggtataa attaaatcac ttttttcaat tgacgcgtga tctaattcta ccgggtaggg
      421 gaggcgcttt tcccaaggca gtctggagca tgcgctttag cagccccgct gggcacttgg
      481 cgctacacaa gtggcctctg gcctcgcaca cattccacat ccaccggtag gcgccaaccg
      541 gctccgttct ttggtggccc cttcgcgcca ccttctactc ctcccctagt caggaagttc
      601 ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact
      661 agtctcgtgc agatggacag caccgctgag caatggaagc gggtaggcct ttggggcagc
      721 ggccaatagc agctttgctc cttcgctttc tgggctcaga ggctgggaag gggtgggtcc
      781 gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc
      841 ccggcattct gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct
      901 ccgggccttt
//
