LOCUS       sequence-147810-         809 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of (-30)dGFP-9xGGS-Cath-NLS-Cas9-6xHis in bacterial 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Zuris JA, Thompson DB, Shu Y, Guilinger JP, Bessen JL, Hu JH, Maeder
            ML, Joung JK, Chen ZY, Liu DR
  TITLE     Cationic lipid-mediated delivery of proteins enables efficient 
            protein-based genome editing in vitro and in vivo.
  JOURNAL   Nat Biotechnol. 2015 Jan;33(1):73-80. doi: 10.1038/nbt.3081. Epub 
            2014 Oct 30.
  PUBMED    25357182
REFERENCE   2  (bases 1 to 809)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.3081"; 
            journalName: "Nat Biotechnol"; date: "2015-01"; volume: "33"; issue:
            "1"; pages: "73-80"
COMMENT     Sequence Label: pET-(-30)dGFP-9xGGS-Cath-NLS-Cas9-6xHis
FEATURES             Location/Qualifiers
     source          1..809
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     44..63
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        44..62
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    63..87
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             102..124
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 cgtccggcgt agaggatcga gatcgatctc gatcccgcga aattaatacg actcactata
       61 ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag
      121 gagatatacc atgggtgcta gcaaaggtga agagctgttt gacggtgtag taccgatctt
      181 agtggaatta gacggcgacg tgaacggtca cgaatttagc gtgcgcggcg agggcgaagg
      241 tgacgctacc gagggtgaat tgaccctgaa gtttatttgc acaacaggcg aattacccgt
      301 tccgtggccc accttagtga ccaccctgac ctccggcgtt cagtgcttca gtgattaccc
      361 agatcatatg gatcaacacg attttttcaa atcagccatg cctgaaggat atgttcaaga
      421 gcgtacaatc agcttcaagg acgatggcac ctataaaacg cgtgcggaag tgaaatttga
      481 aggcgacaca ttagtaaacc gtatcgaact gaaaggtatc gacttcaaag aagacggcaa
      541 cattttaggc cataagctgg aatataactt taattctcat gacgtgtata ttacggccga
      601 taaacaggaa aacggtatca aggcagaatt tgaaattcgc cataacgtgg aggacggcag
      661 cgttcaatta gcggatcatt atcaacaaaa cacgccgatt ggtgatgggc ctgtactgtt
      721 acctgacgat cactacctga gcacggagtc agccctgagc aaagatccga acgaagaccg
      781 cgatcacatg gttctgttag aattcgtga
//
