LOCUS       sequence-147814-         927 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral expression plasmid encoding shBrd9_1061 (targeting murine
            Brd9).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Hohmann AF, Martin LJ, Minder JL, Roe JS, Shi J, Steurer S, Bader G,
            McConnell D, Pearson M, Gerstberger T, Gottschamel T, Thompson D, 
            Suzuki Y, Koegl M, Vakoc CR
  TITLE     Sensitivity and engineered resistance of myeloid leukemia cells to 
            BRD9 inhibition.
  JOURNAL   Nat Chem Biol. 2016 Jul 4. doi: 10.1038/nchembio.2115.
  PUBMED    27376689
REFERENCE   2  (bases 1 to 927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2016 Jul 4. doi: 10.1038/nchembio.2115."
COMMENT     Sequence Label: MSCV-miR30_shBrd9_1061-PGK-NeoR-IRES-GFP
FEATURES             Location/Qualifiers
     source          1..927
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(143..145,146..148,149..862)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(188..209)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(449..468)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     796..817
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg tgtttagtcg
       61 aggttaaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca
      121 cgatgataat atggccacaa ccatggtgag caagggcgag gagctgttca ccggggtggt
      181 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtctggcga
      241 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
      301 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag
      361 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
      421 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
      481 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
      541 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
      601 catggccgac aagcagaaga acggcatcaa ggcgaacttc aagatccgcc acaacatcga
      661 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
      721 cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa
      781 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
      841 catggacgag ctgtacaagt aatgaattaa ttaagaatta tcaagcttat cgataccgtc
      901 gacctgcagc caagcttatc gataaaa
//
