LOCUS       sequence-147818-         979 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral expression plasmid encoding shBrd9_1116 (targeting murine
            Brd9).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 979)
  AUTHORS   Hohmann AF, Martin LJ, Minder JL, Roe JS, Shi J, Steurer S, Bader G,
            McConnell D, Pearson M, Gerstberger T, Gottschamel T, Thompson D, 
            Suzuki Y, Koegl M, Vakoc CR
  TITLE     Sensitivity and engineered resistance of myeloid leukemia cells to 
            BRD9 inhibition.
  JOURNAL   Nat Chem Biol. 2016 Jul 4. doi: 10.1038/nchembio.2115.
  PUBMED    27376689
REFERENCE   2  (bases 1 to 979)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2016 Jul 4. doi: 10.1038/nchembio.2115."
COMMENT     Sequence Label: MSCV-miR30_shBrd9_1116-PGK-NeoR-IRES-GFP
FEATURES             Location/Qualifiers
     source          1..979
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           65..186
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           214..228
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           256..382
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     promoter        412..911
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(447..470)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     844..863
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
ORIGIN
        1 agccctcact ccttctctag gcgccggaat tagatctctc gactagggat aacagggtaa
       61 ttgtttgaat gaggcttcag tactttacag aatcgttgcc tgcacatctt ggaaacactt
      121 gctgggatta cttcttcagg ttaacccaac agaaggctcg agaaggtata ttgctgttga
      181 cagtgagcga aaagatgaaa gaagaaataa atagtgaagc cacagatgta tttatttctt
      241 ctttcatctt tgtgcctact gcctcggaat tcaaggggct actttaggag caattatctt
      301 gtttactaaa actgaatacc ttgctatctc tttgatacat ttttacaaag ctgaattaaa
      361 atggtataaa ttaaatcact tttttcaatt gacgcgtgat ctaattctac cgggtagggg
      421 aggcgctttt cccaaggcag tctggagcat gcgctttagc agccccgctg ggcacttggc
      481 gctacacaag tggcctctgg cctcgcacac attccacatc caccggtagg cgccaaccgg
      541 ctccgttctt tggtggcccc ttcgcgccac cttctactcc tcccctagtc aggaagttcc
      601 cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta
      661 gtctcgtgca gatggacagc accgctgagc aatggaagcg ggtaggcctt tggggcagcg
      721 gccaatagca gctttgctcc ttcgctttct gggctcagag gctgggaagg ggtgggtccg
      781 ggggcgggct caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc
      841 cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct tcctcatctc
      901 cgggcctttc gacctgcagc caatatggga tcggccattg aacaagatgg attgcacgca
      961 ggttctccgg ccgcttggg
//
