LOCUS       sequence-147822-         909 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral expression plasmid encoding shBrd9_1061 (targeting murine
            Brd9).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 909)
  AUTHORS   Hohmann AF, Martin LJ, Minder JL, Roe JS, Shi J, Steurer S, Bader G,
            McConnell D, Pearson M, Gerstberger T, Gottschamel T, Thompson D, 
            Suzuki Y, Koegl M, Vakoc CR
  TITLE     Sensitivity and engineered resistance of myeloid leukemia cells to 
            BRD9 inhibition.
  JOURNAL   Nat Chem Biol. 2016 Jul 4. doi: 10.1038/nchembio.2115.
  PUBMED    27376689
REFERENCE   2  (bases 1 to 909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2016 Jul 4. doi: 10.1038/nchembio.2115."
COMMENT     Sequence Label: MSCV-miR30_shBrd9_1061-PGK-NeoR-IRES-mCherry
FEATURES             Location/Qualifiers
     source          1..909
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           70..191
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           219..233
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           261..387
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     primer_bind     complement(452..475)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     849..868
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
ORIGIN
        1 tttatcagcc ctcctccttc tctaggcgcc ggaattagat ctctcgacta gggataacag
       61 ggtaattgtt tgaatgaggc ttcagtactt tacagaatcg ttgcctgcac atcttggaaa
      121 cacttgctgg gattacttct tcaggttaac ccaacagaag gctcgagaag gtatattgct
      181 gttgacagtg agcgcggacc tgagttcact gtctaatagt gaagccacag atgtattaga
      241 cagtgaactc aggtccatgc ctactgcctc ggaattcaag gggctacttt aggagcaatt
      301 atcttgttta ctaaaactga ataccttgct atctctttga tacattttta caaagctgaa
      361 ttaaaatggt ataaattaaa tcactttttt caattgacgc gtgatctaat tctaccgggt
      421 aggggaggcg cttttcccaa ggcagtctgg agcatgcgct ttagcagccc cgctgggcac
      481 ttggcgctac acaagtggcc tctggcctcg cacacattcc acatccaccg gtaggcgcca
      541 accggctccg ttctttggtg gccccttcgc gccaccttct actcctcccc tagtcaggaa
      601 gttccccccc gccccgcagc tcgcgtcgtg caggacgtga caaatggaag tagcacgtct
      661 cactagtctc gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag gcctttgggg
      721 cagcggccaa taacagcttt gctccttcgc tttctgggct cagaggctgg gaaggggtgg
      781 gtccgggggc gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa ggtcctccgg
      841 aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct cctcttcctc
      901 atctccggg
//
