LOCUS       sequence-147825-         993 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral expression plasmid encoding shBrd9_1116 (targeting murine
            Brd9).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 993)
  AUTHORS   Hohmann AF, Martin LJ, Minder JL, Roe JS, Shi J, Steurer S, Bader G,
            McConnell D, Pearson M, Gerstberger T, Gottschamel T, Thompson D, 
            Suzuki Y, Koegl M, Vakoc CR
  TITLE     Sensitivity and engineered resistance of myeloid leukemia cells to 
            BRD9 inhibition.
  JOURNAL   Nat Chem Biol. 2016 Jul 4. doi: 10.1038/nchembio.2115.
  PUBMED    27376689
REFERENCE   2  (bases 1 to 993)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2016 Jul 4. doi: 10.1038/nchembio.2115."
COMMENT     Sequence Label: MSCV-miR30_shBrd9_1116-PGK-NeoR-IRES-mCherry
FEATURES             Location/Qualifiers
     source          1..993
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     8..24
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     ncRNA           80..201
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           229..243
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           271..397
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     promoter        427..926
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(462..485)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     859..878
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
ORIGIN
        1 atcctccctt tatccagccc tcactccttc tctaggcgcc ggaattagat ctctcgacta
       61 gggataacag ggtaattgtt tgaatgaggc ttcagtactt tacagaatcg ttgcctgcac
      121 atcttggaaa cacttgctgg gattacttct tcaggttaac ccaacagaag gctcgagaag
      181 gtatattgct gttgacagtg agcgaaaaga tgaaagaaga aataaatagt gaagccacag
      241 atgtatttat ttcttctttc atctttgtgc ctactgcctc ggaattcaag gggctacttt
      301 aggagcaatt atcttgttta ctaaaactga ataccttgct atctctttga tacattttta
      361 caaagctgaa ttaaaatggt ataaattaaa tcactttttt caattgacgc gtgatctaat
      421 tctaccgggt aggggaggcg cttttcccaa ggcagtctgg agcatgcgct ttagcagccc
      481 cgctgggcac ttggcgctac acaagtggcc tctggcctcg cacacattcc acatccaccg
      541 gtaggcgcca accggctccg ttctttggtg gccccttcgc gccaccttct actcctcccc
      601 tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg caggacgtga caaatggaag
      661 tagcacgtct cactagtctc gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag
      721 gcctttgggg cagcggccaa tagcagcttt gctccttcgc tttctgggct cagaggctgg
      781 gaaggggtgg gtccgggggc gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa
      841 ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct
      901 cctcttcctc atctccgggc ctttcgacct gcagccaata tgggatcggc cattgaacaa
      961 aatggattgc acgcaggttc tccggccgct tgg
//
