LOCUS       sequence-147834-         976 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral expression plasmid encoding inducible shBrd9_1116 
            (targeting murine Brd9).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 976)
  AUTHORS   Hohmann AF, Martin LJ, Minder JL, Roe JS, Shi J, Steurer S, Bader G,
            McConnell D, Pearson M, Gerstberger T, Gottschamel T, Thompson D, 
            Suzuki Y, Koegl M, Vakoc CR
  TITLE     Sensitivity and engineered resistance of myeloid leukemia cells to 
            BRD9 inhibition.
  JOURNAL   Nat Chem Biol. 2016 Jul 4. doi: 10.1038/nchembio.2115.
  PUBMED    27376689
REFERENCE   2  (bases 1 to 976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2016 Jul 4. doi: 10.1038/nchembio.2115."
COMMENT     Sequence Label: MSCV-TRE-dsRed-miR30_shBrd9_1116-PGK-Venus-IRE...
FEATURES             Location/Qualifiers
     source          1..976
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     7..23
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     protein_bind    join(89..107,108..130,131..149,150..172,173..191,192..214,
                     215..233,234..256,257..275,276..298,299..317,318..340,
                     341..359)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        392..430
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     427..451
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     complement(709..729)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
ORIGIN
        1 tcctcccttt atccagccct cactccttct ctaggcgccg gaattgaaga tctgggggat
       61 cgatcctcta gagcatcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg
      121 agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc
      181 agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga
      241 aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc
      301 cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa
      361 aagtgaaagt cgagctcggt acccgggtcg aggtaggcgt gtacggtggg aggcctatat
      421 aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
      481 cctccataga agacaccggg accgatccag cctctcgacg gatccaccat ggcctcctcc
      541 gagaacgtca tcaccgagtt catgcgcttc aaggtgcgca tggagggcac cgtgaacggc
      601 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcca caacaccgtg
      661 aagctgaagg tgaccaaggg cggccccctg cccttcgcct gggacatcct gtccccccag
      721 ttccagtacg gctccaaggt gtacgtgaag caccccgccg acatccccga ctacaagaag
      781 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg
      841 gcgaccgtga cccaggactc ctccctgcag gacggctgct tcatctacaa ggtgaagttc
      901 atcggcgtga acttcccctc cgacggcccc gtgatgcaga agaagaccat gggctgggag
      961 gcctccaccg agcgcc
//
