LOCUS       sequence-147840-         984 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral expression plasmid encoding inducible shBrd9_1116 
            (targeting murine Brd9).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 984)
  AUTHORS   Hohmann AF, Martin LJ, Minder JL, Roe JS, Shi J, Steurer S, Bader G,
            McConnell D, Pearson M, Gerstberger T, Gottschamel T, Thompson D, 
            Suzuki Y, Koegl M, Vakoc CR
  TITLE     Sensitivity and engineered resistance of myeloid leukemia cells to 
            BRD9 inhibition.
  JOURNAL   Nat Chem Biol. 2016 Jul 4. doi: 10.1038/nchembio.2115.
  PUBMED    27376689
REFERENCE   2  (bases 1 to 984)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2016 Jul 4. doi: 10.1038/nchembio.2115."
COMMENT     Sequence Label: MSCV-TRE-dsRed-miR30_shBrd9_1116-PGK-Venus-IRE...
FEATURES             Location/Qualifiers
     source          1..984
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           119..240
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           268..282
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           310..436
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     promoter        459..958
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(494..517)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     891..910
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
ORIGIN
        1 tcgtggagca gtacgagcgc accgagggcc gccaccacct gttcctgtag cggccgcgac
       61 tctagaattt actagtcata tgataagatc tctcgactag ggataacagg gtaattgttt
      121 gaatgaggct tcagtacttt acagaatcgt tgcctgcaca tcttggaaac acttgctggg
      181 attacttctt caggttaacc caacagaagg ctcgagaagg tatattgctg ttgacagtga
      241 gcgaaaagat gaaagaagaa ataaatagtg aagccacaga tgtatttatt tcttctttca
      301 tctttgtgcc tactgcctcg gaattcaagg ggctacttta ggagcaatta tcttgtttac
      361 taaaactgaa taccttgcta tctctttgat acatttttac aaagctgaat taaaatggta
      421 taaattaaat cacttttttc aattctagca attctaccgg gtaggggagg cgcttttccc
      481 aaggcagtct ggagcatgcg ctttagcagc cccgctgggc acttggcgct acacaagtgg
      541 cctctggcct cgcacacatt ccacatccac cggtaggcgc caaccggctc cgttctttgg
      601 tggccccttc gcgccacctt ctactcctcc cctagtcagg aagttccccc ccgccccgca
      661 gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt ctcactagtc tcgtgcagat
      721 ggacagcacc gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc aatagcagct
      781 ttgctccttc gctttctggg ctcagaggct gggaaggggt gggtccgggg gcgggctcag
      841 gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac
      901 gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac
      961 ctgcagccca agcttccacc atgg
//
