LOCUS       sequence-147843-        1022 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral expression plasmid encoding shBRD9_508 (targeting human 
            BRD).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Hohmann AF, Martin LJ, Minder JL, Roe JS, Shi J, Steurer S, Bader G,
            McConnell D, Pearson M, Gerstberger T, Gottschamel T, Thompson D, 
            Suzuki Y, Koegl M, Vakoc CR
  TITLE     Sensitivity and engineered resistance of myeloid leukemia cells to 
            BRD9 inhibition.
  JOURNAL   Nat Chem Biol. 2016 Jul 4. doi: 10.1038/nchembio.2115.
  PUBMED    27376689
REFERENCE   2  (bases 1 to 1022)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2016 Jul 4. doi: 10.1038/nchembio.2115."
COMMENT     Sequence Label: MSCV-miRE_shBRD9_508-SV40-GFP
FEATURES             Location/Qualifiers
     source          1..1022
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     7..23
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     ncRNA           228..242
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     primer_bind     complement(440..460)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        445..774
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      625..760
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(826..847)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 tcctcccttt atccagccct cactccttct ctaggcgccg gaattagatc tctcgactag
       61 ggataacagg gtaattgttt gaatgaggct tcagtacttt acagaatcgt tgcctgcaca
      121 tcttggaaac acttgctggg attacttcga cttcttaacc caacagaagg ctcgagaagg
      181 tatattgctg ttgacagtga gcgactcctg gatattcaat gataatagtg aagccacaga
      241 tgtattatca ttgaatatcc aggagctgcc tactgcctcg gacttcaagg ggctagaatt
      301 cgagcaatta tcttgtttac taaaactgaa taccttgcta tctctttgat acatttttac
      361 aaagctgaat taaaatggta taaattaaat cacttttttc aattgacgcg taattcgcca
      421 gcacagtggt cgaccctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
      481 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
      541 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
      601 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
      661 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
      721 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc
      781 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
      841 ggcgacgtaa acggccacaa gttcagcgtg tctggcgagg gcgagggcga tgccacctac
      901 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
      961 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
     1021 ca
//
