LOCUS       sequence-147847-        1019 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral expression plasmid encoding shBRD9_561 (targeting human 
            BRD).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Hohmann AF, Martin LJ, Minder JL, Roe JS, Shi J, Steurer S, Bader G,
            McConnell D, Pearson M, Gerstberger T, Gottschamel T, Thompson D, 
            Suzuki Y, Koegl M, Vakoc CR
  TITLE     Sensitivity and engineered resistance of myeloid leukemia cells to 
            BRD9 inhibition.
  JOURNAL   Nat Chem Biol. 2016 Jul 4. doi: 10.1038/nchembio.2115.
  PUBMED    27376689
REFERENCE   2  (bases 1 to 1019)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2016 Jul 4. doi: 10.1038/nchembio.2115."
COMMENT     Sequence Label: MSCV-miRE_shBRD9_561-SV40-GFP
FEATURES             Location/Qualifiers
     source          1..1019
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           223..237
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     primer_bind     complement(435..455)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        440..769
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      620..755
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(821..842)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ccctttatcc agccctcctc cttctctagg cgccggaatt agatctctcg actagggata
       61 acagggtaat tgtttgaatg aggcttcagt actttacaga atcgttgcct gcacatcttg
      121 gaaacacttg ctgggattac ttcgacttct taacccaaca gaaggctcga gaaggtatat
      181 tgctgttgac agtgagcgcg acaaaattgt agctaatgaa tagtgaagcc acagatgtat
      241 tcattagcta caattttgtc ttgcctactg cctcggactt caaggggcta gaattcgagc
      301 aattatcttg tttactaaaa ctgaatacct tgctatctct ttgatacatt tttacaaagc
      361 tgaattaaaa tggtataaat taaatcactt ttttcaattg acgcgtaatt cgccagcaca
      421 gtggtcgacc ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
      481 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
      541 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
      601 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
      661 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
      721 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcatggt
      781 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
      841 cgtaaacggc cacaagttca gcgtgtctgg cgagggcgag ggcgatgcca cctacggcaa
      901 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
      961 gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagc
//
